FastQCFastQC Report
Tue 22 Aug 2023
SRR7825646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7825646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45444886
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTCCCGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA560239312.32788437405256Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGTCCCGGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC28267686.220211444693689TruSeq Adapter, Index 5 (100% over 35bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC16648543.6634573139868807Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG12262052.6982243942695776TruSeq Adapter, Index 5 (100% over 49bp)
CGGTCCCGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6667001.467051760235464Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGTCCCGGGCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6508091.4320841293341566Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA5552551.2218206466619808Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGTCCCGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC5518611.2143522595699767Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4527470.9962551121813795Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTTCGCCGCGCCGGGGCCGTGGAACAGATCGGAAGAGCACACGTCTGAA3700680.8143226500777226Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGTCCCGGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3017880.6640747211908508Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGCCGGGGCCGTGGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT2377530.5231677773380266Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1960150.43132465994083474Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTTCTGGTGTCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAAC1700390.374165313122361Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAG1686800.37117487763089557Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1588290.349498071136101TruSeq Adapter, Index 5 (100% over 35bp)
CTAAATCACTTGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA1588210.3494804673951652Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGCCGGGGCCGTGGAACCAGATCGGAAGAGCACACGTCTGAACTCCAG1454040.31995679337824723Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGTCCCGGGCGCACCACCGGCCCGTAGATCGGAAGAGCACACGTCTGA1428830.3144094145158599Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTCCCGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1279460.281541029721144Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGTCCCGGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA1209380.26612015266140177TruSeq Adapter, Index 5 (100% over 36bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1159560.2551574229936455Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCGCCGGGGCCGTGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG1064580.2342573815676422Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1020470.22455111890917717Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCCCGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC993640.21864726429284032TruSeq Adapter, Index 5 (100% over 35bp)
GCGCCGGGGCCGTGGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC979200.21546978905393224Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGGTCCCGGGCACACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA943400.20759211498517127Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGCGCCGGGGCCGTGGAACAGATCGGAAGAGCACACGTCTGAACTCCA930330.2047161038097884Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA909980.2002381522092497Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGCGGACGAGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGA896140.19719270502735997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTCCCGGGCGCACCACCAGATCGGAAGAGCACACGTCTGAACTCCAG887140.19521228417208483Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT860020.18924461599485584Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGTCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG838970.18461263166112904No Hit
CACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA829340.18249358134598467Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGTCCCGGGCGCATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA805010.17713984363389093Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAAATCACTTGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAAC800000.1760374093577878Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT774910.1705164361068042Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC749160.1648502319931004Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGTCCCGGGCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA732490.1611820524756075Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGTGGAACCAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC727120.16000040136529334TruSeq Adapter, Index 5 (100% over 35bp)
GCGCCGGGGCCGTGGAACCAGATCGGAAGAGCACACGTCTGAACTCCAGT718520.15810799921469712Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCCCGGACTTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC717130.15780213421593797TruSeq Adapter, Index 5 (100% over 35bp)
CCGCGCCGGGGCCGTGGAACCAGATCGGAAGAGCACACGTCTGAACTCCA688390.15147799028475945Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTTCTGGTGTCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAACT685030.15073863316545671Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCCGGACTTCGCCGCGCCGGGGCCGTGGAACAGATCGGAAGAGCACAC639750.14077491579580595No Hit
ACTTCGCCGCGCCGGGGCCGTGGAAAGATCGGAAGAGCACACGTCTGAAC631900.13904754871648264Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTCGCCGCGCCGGGGCCGTGGAACCAGATCGGAAGAGCACACGTCTGA611050.1344595737350953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA609880.13420211902390955TruSeq Adapter, Index 5 (100% over 36bp)
TCCCGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA602280.13252976363501054Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTCCTGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA564800.12428241100659819Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGGCCGTGGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA554350.1219829223468621Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGACTCCCGGTATGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA535130.11775362358704122Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGCGCCGGGGCCGTGGAACCAGATCGGAAGAGCACACGTCTGAACTCC520760.11459155162145196Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTCTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC481860.10603173259142953Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTC455790.10029511351398264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)