FastQCFastQC Report
Tue 22 Aug 2023
SRR7825647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7825647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44413825
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1052780723.703896253024816TruSeq Adapter, Index 9 (100% over 49bp)
TCGGTCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG4370100.9839503803151384No Hit
TCCCGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA3902640.8786993689464937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCGGACTTCGCCGCGCCGGGGCCGTGGAACAGATCGGAAGAGCACAC3894120.8767810473428037No Hit
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC3836910.8638999230532385Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTG2950200.6642526285452784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCCCGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2810090.632706144989764Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGCGGACGAGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGA2430970.547345336727922Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG1918060.4318610252550867TruSeq Adapter, Index 9 (100% over 50bp)
CGGGCGGACGAGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG1865090.4199345586650103Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCCCGGGCGCACCACCGGCCCGTAGATCGGAAGAGCACACGTCTGA1837170.41364822777592336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1810460.40763433457937925TruSeq Adapter, Index 9 (100% over 50bp)
CGCCCGGACTTCGCCGCGCCGGGGCCGTGGAACCAGATCGGAAGAGCACA1633060.36769181668095463No Hit
AGCCCGTAGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT1630990.3672257455871004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGTCCCGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1507680.3394618680106926No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1377030.31004535186960364TruSeq Adapter, Index 9 (100% over 50bp)
ACTTCGCCGCGCCGGGGCCGTGGAACAGATCGGAAGAGCACACGTCTGAA1336960.3010233862991985Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1280340.2882751035291376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTCTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1254000.28234451772618097Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT1183410.2664508179603986Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGTCCCGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1035110.23306031399006952Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGTCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1004000.22605573827518796No Hit
ACTTCGCCGCGCCGGGGCCGTGGAACCAGATCGGAAGAGCACACGTCTGA983310.22139727888782376Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTCTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA924360.20812438469327965Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAG893150.20109729346661767Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTGCATAATTTCTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA841170.1893937304431672Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC797120.1794756475039022Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC790950.1780864404270517TruSeq Adapter, Index 9 (98% over 50bp)
TTGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC729060.16415159018616388No Hit
CTCCCGGGGCTACGCCTGTCTGAGGGTCGTTAGATCGGAAGAGCACACGT711800.16026541285286733No Hit
ATATGGTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTG684640.15415019985331146Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA667270.15023925545705646Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT602010.13554563246916923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTTCGCCGCGCCGGGGCCGTGGAACCAGATCGGAAGAGCACACGTCTG580030.13059672297983793Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATG573570.12914222091882427TruSeq Adapter, Index 9 (97% over 49bp)
GCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCC572390.12887653787981557Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG572280.12885177081685714TruSeq Adapter, Index 9 (97% over 49bp)
CACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT506130.11395775977412438Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT468220.1054221292581758Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA463100.10426933505501947Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCCGGACTTCGCCGCGCCGGGGCCGTGGAACAGATCGGAAGAGCACA459690.10350155610330791No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC455690.10260093563209202Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT450550.10144363832657961TruSeq Adapter, Index 9 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)