FastQCFastQC Report
Fri 7 Jul 2023
SRR7874134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7874134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104344429
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA74393807.129637941667206Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC6529550.6257689138343936Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA6027320.5776369718789682No Hit
GCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA5460580.5233226203192889Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC4851460.46494671986752645No Hit
TACTTGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA4264850.40872809798019977Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC4029910.38621228163508375No Hit
ATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4019080.38517437284553063Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3852430.3692032278982522Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3529690.33827297095084974Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3379500.3238792940253667Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACTTGGATAACTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3369380.32290942911767717No Hit
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3360500.3220584014121156Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3033560.29072563136073126Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3001720.2876741986867358No Hit
CGCTGCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2953470.2830500898136114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2804680.26879058392278904Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2710860.25979920787146193Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2703680.2591111021365597Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2466530.23638348722958652Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTTGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC2374460.2275598249715852Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2260150.21660475999154682Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2238320.21451265021537472Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2223880.21312877182930387Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCTGCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2220060.21276267657758713Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGCATGATGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2173440.20829478112338895No Hit
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2172260.20818169410846077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2133310.20444886425129605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2114500.2026461805641775No Hit
CAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2021390.193722848394714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1904470.18251765027148695Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1900640.18215059665523686Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1879390.18011407202199553Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCCTGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1873470.17954672021828783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1867590.1789832018727133Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1778740.17046813299443137No Hit
AATTCTAGAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1700020.1629238873883722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1661690.15925047613227153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1582730.15168322977741341Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1570460.15050731649506655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1563650.14985467024789603No Hit
GCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1525150.1461649667947294Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1483850.14220692127224155Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1477670.14161465199066833Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1446280.13860634572067093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1444690.13845396575987778Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1440280.1380313270006969Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1422410.13631872957970761Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1365570.13087138557248704No Hit
AGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1352720.12963988714720937Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1343780.12878310925444808Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1335030.1279445402878193Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCCACGCGCGCGTCCCCCCTGTAGGCACCATCAATAGATCGGAAGAG1264370.12117273649559192No Hit
TAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1249600.11975723207992253Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1248890.11968918819805896No Hit
GTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1241030.11893591367489298No Hit
GACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1229650.11784529483600892Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCCGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1222270.11713802181044088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGC1218660.11679205221392318No Hit
AATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1217730.11670292431232722Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1212480.11619978293234993Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACTTGGATAACTGTGGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1201460.11514366521666432No Hit
ACTTGGATAACTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1197680.11478140342308069Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1194700.11449581079216026No Hit
AGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1192600.11429455424016935Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1186610.11372049388472863Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1179130.11300363721382768Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1170610.11218711063146457Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1162990.11145683685709756Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1161760.1113389580195029Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1142210.10946535535692088Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGC1137370.10900150692280851No Hit
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1110530.10642925651545805Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1072060.1027424281558913Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1064920.1020581558791222Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1049720.10060144178852136Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGGTAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1048050.10044139491146191Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)