FastQCFastQC Report
Fri 7 Jul 2023
SRR7874135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7874135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77075024
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA50358176.533656090720127Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC8637221.120625015958477Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT8396001.089328236861788Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA5090780.6604967129170145Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC4485680.581988790558145No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC3984920.5170183274934822No Hit
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3538510.4590994353761083Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3490820.4529119575752581Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3116680.4043696437901855Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3069100.39819643779806024Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3062800.39737905238926685Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2932750.38050588216488906No Hit
GTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2835550.3678947930006483Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2683180.3481257430422597Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2653780.3443112778012239Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC2590410.3360894185384879Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGC2562870.33251627660861965No Hit
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2519350.32686983010216125Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2505350.32505341808262034Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2430000.31527722910602013Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2245880.2913888161747442Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2158790.2800894359760433Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2147390.2786103576172743Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2066300.2680894397126623Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1840400.23878033434021373Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1820410.23618675746374077No Hit
GGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1775830.23040278261865998Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA1765960.22912221214488365No Hit
TACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1744070.22628212220861585Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1710750.22195906160210863Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1647080.2136982792246682No Hit
TACTTGGATAACTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1548840.2009522565961186No Hit
CGCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1527970.19824450524984594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1494160.19385787022265477No Hit
ATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1455500.18884197817440834Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1439660.18678683771801355Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1418240.18400772732811604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1358590.17626851468771518Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1337860.17357892746163792Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1328480.17236193140854553Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1323320.17169245383562903Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1306830.16955297996404128No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT1179570.15304179470641488No Hit
AGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1164750.1511189928400152Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1150270.14924030383694722Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1135080.1472694967957454Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1130970.14673625012429448Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA1089850.14140118853547162No Hit
GAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1085050.14077841870020047Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1079040.13999865896895472Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1072700.13917608381153407Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1067150.1384560061895018Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1058640.1373518871690523Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1053780.13672133271084028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1053600.13669797884201762Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1052690.13657991206074746Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1048270.1360064448374353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1033390.1340758583480947No Hit
AGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1025020.13298990344784062Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1022230.1326279184810893Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG997120.12937005378032707No Hit
GTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC995900.12921176644719565Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT988200.12821273983644818Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA988110.12820106290203684No Hit
TAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA986790.12802980119733728Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG950890.1233720018043718Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT943110.1223625956963698Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA939950.12195260555481631Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA930230.12069149663839222Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT926860.1202542603165456Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT923940.11987540866675567Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACC921230.11952380319725882No Hit
AGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC921100.11950693651422023Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT888670.11529934781466951Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA883400.11461559843302806Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC880680.11426269552637441No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA871490.1130703507792615No Hit
CCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG856800.11116441559590043Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC851320.11045342003396585Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA844370.10955170120997952Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA838440.10878232097598828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA824600.10698666795095646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT821150.10653905213185533Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA807940.10482513764770285Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT806320.10461495282829883Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA798870.10364836214647173Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG791760.10272588432797633No Hit
GAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA789410.10242098659612484Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG786570.1020525144435894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA785330.10189163223614436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)