FastQCFastQC Report
Thu 13 Jul 2023
SRR7874136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7874136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127289140
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2325189418.266989626923397Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC19243641.5118053276186798Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA10020780.7872454790722917Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT8818470.6927904454378433Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC7533990.5918800299852761Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT7248670.5694649205737425Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT6742790.5297223313787806Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT5519350.43360729752750315Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC4885570.3838167183783314Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4871230.38269014937173745Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT4799300.3770392352403355Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4256750.3344158032649132Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA4222070.3316912974665396Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3983230.3129277171642451Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3774710.2965461154030894Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA3714920.2918489354237133Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT3574120.28078750473135416No Hit
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3512460.27594341512559517Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3071750.24132066569072583Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3066830.24093414410687355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2947090.23152721434051643Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2944480.23132216935396058Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2773890.21792039760815418Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2642360.207587230143907Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2641610.2075283091707588No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG2466620.19378086771581615No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT2435870.1913651078167391No Hit
ACGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2339090.18376194544169283No Hit
GCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC2298970.1806100661847507Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2275080.17873323678673608Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2273640.1786201085182915Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC2229540.1751555552971762Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG2197860.17266673339139538Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG2090990.1642708875242617Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT2086850.16394564375248352No Hit
TCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA2074080.16294241598301315Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2044380.16060914544634367No Hit
CGCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2039520.16022733754034318Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2017250.15847777744432873Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2008610.15779900783366121Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1880260.14771566529556251No Hit
GGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1863590.14640604846572142Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1846090.14503122575892963Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1756580.1379992040169334Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTTGGATAACTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1737990.13653874949583286No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1712680.13455036305532428No Hit
ATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1707540.13414655798601516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1693760.13306398330603852No Hit
GAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1663160.13066000760159113Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1657530.13021770749649184Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC1655480.13005665683655337No Hit
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1626910.12781216056609387Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1626000.12774066978534068No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC1593050.12515207503169556No Hit
TTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1551660.12190042292688913Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1545790.12143926811038239Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1514830.11900701033882388Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1480520.11631157222053665Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1471220.11558095215349871Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1460820.11476391465917674Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1453070.11415506460331179Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1432290.11252256084061844Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1427340.1121336824178402No Hit
CACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1423880.11186186032838309Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1414980.11116266478035754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1397200.10976584491025707Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1376440.10813491237351436Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1360870.1069117129709573No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1342190.10544418793307897No Hit
ATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1333920.10479448600249792Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1318000.10354379014580505Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1310900.10298600493333523Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1295810.10180051495359305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)