FastQCFastQC Report
Mon 10 Jul 2023
SRR7874137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7874137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89273230
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1502947816.83536934868381Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG10780311.207563566368104Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC8978891.0057763116670024Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC5374460.6020236973614599Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4943300.5537270243274496Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC4908150.5497896737913482Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA4753780.5324978159746209Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4010090.4491928879463642Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3897270.4365552809055973Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3366920.37714777431039515Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3236090.3624927651883997Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2972580.33297551796882446Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2882350.3228683447434354Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2861730.32055858178313923Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2602370.2915061995628477Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2216960.24833424308720542Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2181110.24431848158736946No Hit
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2090080.234121695831998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2061380.23090684631887967Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2051190.22976540671822898Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC2046620.2292534951407046TruSeq Adapter, Index 6 (100% over 36bp)
GGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2010820.2252433344239925Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1943130.21766099423085736No Hit
GGGCGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1804210.20209977839941493No Hit
GTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1792150.20074886950993037Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1686190.1888796899137625Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1646150.18439458278814377No Hit
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1613970.18078991876960204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1576620.1766061337760491Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1570160.17588251259644128Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1546430.17322438092583858No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT1542900.17282896563729128No Hit
GCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1536750.17214006931305162Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1526760.1710210328448965No Hit
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1355320.15181706766966985Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1311650.146925343689256Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1306000.14629245519625536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA1279050.14327363309247354No Hit
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1265240.14172669679365246Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1255610.14064798596398942No Hit
TACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1188890.13317430096345792Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCACGCAGTTTTATCCGGTACTGTAGGCACCATCAATAGATCGGAAG1183030.1325178891813369No Hit
GCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1147840.1285760580187364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1133890.12701343952716845Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1096550.12283077469024029Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1066490.11946358387615189Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1053430.11800065932418935Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1050450.11766685265000494No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1030990.11548702785818324No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA1025640.11488774406392599No Hit
TGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1009170.1130428461029135Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT979190.10968461654182335No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA978140.10956700009622145No Hit
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC974640.10917494527754848Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC966440.10825641684522896No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA955010.10697607782310554No Hit
AGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA912410.10220421060154317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT906680.10156236085554426Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)