FastQCFastQC Report
Thu 13 Jul 2023
SRR7874138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7874138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103935152
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA98379859.465503066758396Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG14298421.3757058824525508Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA9775740.9405614762558869Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT9286050.8934465213463102Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT8855110.8519841294887411Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC8285340.7971643703373811Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC7629710.7340836909537594Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6696040.6442517157236659Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT6160760.5927503718857312Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC6158250.5925088751493817Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT5469960.5262858517780394Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC5376630.5173062141670798Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4975250.4786879033957635Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4739050.4559621945807132Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT4678830.45016819718510637TruSeq Adapter, Index 9 (100% over 36bp)
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4594880.4420910453856844Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4464860.4295813220150965Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4303400.41404663554059173Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4040150.38871834237563824Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3465260.3334059683676607Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3376360.32485255806428226Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3131920.301334047214363Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3128320.30098767739330384Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3102940.2985457701548365Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2618310.2519176572715264Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2556350.24595624779574096Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG2535800.24397905340052803Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2460160.23670143860471765Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2430310.2338294555051019Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2250840.2165619577869093No Hit
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2199530.21162522569842399Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2128760.20481617229943533Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2059670.19816875815027432No Hit
ATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1911160.1838800408931908Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1846440.17765308122125997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1834420.176496590874279No Hit
AATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1783240.17157236658488748Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT1765580.16987323018491376No Hit
GCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1764440.16976354640824504Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1760620.16939600954256553Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1741690.16757468156682928Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1725380.16600543384975278No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1719720.16546086351997638No Hit
AGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1694010.16298720571457864Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGCGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1630290.15685645988183095No Hit
ATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1578260.15185045382913376Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1566360.15070550914285477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1559970.1500907027104747No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA1508450.14513376571576092TruSeq Adapter, Index 9 (100% over 35bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1488440.1432085267937069No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1467850.1412274838449267No Hit
ATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1463390.14079837012217003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1406040.13528050644501874Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1392900.13401625659815267No Hit
CACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1391950.1339248534509287Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1309000.1259439154906898Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1291630.1242726811040792Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1283680.12350778108257349Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1261400.12136413674557382Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1238890.11919836322556203No Hit
TAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1238520.1191627641050643Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACTTGGATAACTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1215900.11698640706274234No Hit
CTGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA1214160.11681899498256375TruSeq Adapter, Index 9 (100% over 35bp)
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1164980.11208719837153844No Hit
ATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1156690.11128958564471046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1155990.11122223595728228No Hit
GAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1129880.10871009261621131Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1117110.10748144188984302Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1105070.10632302726607838No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1092580.10512131641468134No Hit
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1090160.10488847892385822No Hit
GATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1077770.10369638945637949Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1077760.10369542731798766Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1057330.10172977858347673Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG1054710.101477698324817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)