FastQCFastQC Report
Tue 11 Jul 2023
SRR7874139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7874139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92046158
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1116422012.128936440780071Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC11602761.2605371318159744Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC9806131.0653491914350188Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC9213801.0009977820041114Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT8273500.8988425133398833Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA8248570.8961340895944837Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC7627920.828705962936552Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT7379760.8017455763878814Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC6269690.6811463005332608Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT5584350.6066901782038528Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT5354620.5817320479579386Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4972220.5401876741015089Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4794240.5208517231104856Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT4485380.48729681905897687Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4428230.4810879776209671Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3420130.3715668393242443Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG3166520.3440143585352036Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3140510.3411886023531802Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3011630.32718693158273915Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC2914600.3166454812812502No Hit
GGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2863900.3111373752286326Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT2731390.2967413371017615No Hit
GCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2674810.2905944211164142Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT2552660.2773239052519715TruSeq Adapter, Index 12 (100% over 36bp)
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2541220.2760810505529193Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2541130.2760712728498674Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2511140.27281312491065624Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2344980.25476131225379334Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG2232970.24259241759987418Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2113940.22966086210790024Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2097730.2278997891470929Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA1915370.20808798994087294No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1780800.19346815105525644No Hit
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1734180.18840330087432872Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC1716810.18651620418529583No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1664840.18087012387850018No Hit
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1645560.17877552260247517Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1634960.1776239264652415Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1626480.17670264955545456No Hit
TGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1619890.17598670440975928Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1561630.169657271300775Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1518350.1649552825442209No Hit
CACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1478860.1606650437273004Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1395400.15159785376376056Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1369870.14882424533134778Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1336060.14515108821815248Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1333650.14488926305864935Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1318840.14328028770087287Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1287380.13986243727847936Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1249250.1357199504187888Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1242750.1350137829761455Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1232660.13391759382287308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1183540.12858114077939028Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACTTGGATAACTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1157220.12572170584241005No Hit
GAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1114660.12109793871027186Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1100380.11954654315935707Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1095060.1189685722678398Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1093840.11883603007091291Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1084190.11778764302145019Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1068440.11607654498735297No Hit
TAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1062080.11538558730501278Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1056770.11480870282494571Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCCGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1033300.11225889515127833Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1020580.11087697978659794No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT1019370.11074552400112125TruSeq Adapter, Index 12 (100% over 35bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA975200.10594684462549757No Hit
ATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT944400.1026006973588186Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT928980.10092545090257868TruSeq Adapter, Index 12 (100% over 35bp)
ATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC924250.10041157828662442Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT923210.10029859149580148Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)