FastQCFastQC Report
Fri 7 Jul 2023
SRR7883725.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7883725.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Total Bases33.5 Mbp
Sequences flagged as poor quality0
Sequence length20-60
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG109891.0989No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT72070.7207No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT55240.5524No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT54910.5491No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT53640.5364No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC47040.4704No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC46170.46169999999999994No Hit
ACCTCTAGCATTACAAGTATTAGAGGC40220.40220000000000006No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT39870.3987No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGAC37750.3775No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC36580.36579999999999996No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC36070.3607No Hit
TAACCTAGACTTACAAGTCAAAGTAAAAT34870.3487No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC34030.34030000000000005No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAAC31880.3188No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT30530.3053No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCG28810.28809999999999997No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG28440.28440000000000004No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATT26290.26289999999999997No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCC25770.2577No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC25680.2568No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG25220.2522No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG25220.2522No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT22280.2228No Hit
TTTCCATAGTGTAGCGGTTATCACGTCTGCT22110.2211No Hit
AAAAGCATCTGGCCTACACCCAGAAGATT21750.21749999999999997No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG20860.2086No Hit
AGAGTTGCGCAGCGGAAGCGTGCTGGGC19890.1989No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC18400.184No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT18270.1827No Hit
GGAGGCGTGGGTTCGTATCCCACTTCTGACAC18070.1807No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGC17820.1782No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG17020.1702No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC17000.16999999999999998No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGAT16470.1647No Hit
TGCCGCTGGTGTAGTGGTATCATGCAAGATT16110.1611No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGG15740.1574No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT15280.15280000000000002No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT15050.1505No Hit
TATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG14960.14959999999999998No Hit
AACCTAGACTTACAAGTCAAAGTAAAAT14830.1483No Hit
CCGCCGCTGGTGTAGTGGTATCATGCAAGATT14790.1479No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC14760.1476No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT14660.1466No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC14620.1462No Hit
TGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT14210.1421No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT14130.1413No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC14070.1407No Hit
TTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGG14030.1403No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC13780.1378No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT13270.1327No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT13260.1326No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC13170.1317No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT12930.1293No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAA12830.1283No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAT12770.12769999999999998No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC12630.1263No Hit
TCCGCTGGTGTAGTGGTATCATGCAAGATT12340.12340000000000001No Hit
GCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC12310.12310000000000001No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC12020.1202No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCG11970.11969999999999999No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC11940.11939999999999999No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG11830.1183No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCC11690.11689999999999999No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC11410.11410000000000001No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTT11310.1131No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT11240.1124No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCG11170.1117No Hit
GCGAAAGGTCCCCGGTTCGTAACCGGGCGGAAA11150.11150000000000002No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGAT11070.11069999999999999No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT10660.10660000000000001No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC10500.105No Hit
AATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG10350.1035No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC10150.1015No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT10130.1013No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGC10040.10039999999999999No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGATT10010.1001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)