FastQCFastQC Report
Fri 7 Jul 2023
SRR7883731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7883731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Total Bases33.4 Mbp
Sequences flagged as poor quality0
Sequence length20-60
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCACTTCCACCGTGGCGGTGCGCCGCGAC134811.3481No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT77140.7714000000000001No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC63210.6321No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG46780.4678No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC46380.4638No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC44720.44720000000000004No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT42560.42560000000000003No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC37510.3751No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC36530.3653No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGAC33170.3317No Hit
TACGGACCAAGGAGTCTAACGCGTGCGCGAGTC32160.3216No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC31450.3145No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC31000.31No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC29820.29819999999999997No Hit
TGACCGGCTCCGGGACGGCCGGGAAGGCCCGG29160.2916No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC28880.2888No Hit
TCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGAC25510.2551No Hit
AGAGTTGCGCAGCGGAAGCGTGCTGGGC24770.2477No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC23600.23600000000000002No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCC22930.2293No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCC22920.22920000000000001No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCC22730.22729999999999997No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT21030.2103No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC20950.20950000000000002No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC20800.208No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG20670.2067No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAC20450.2045No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG20260.2026No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC19310.1931No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGC19020.1902No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT18790.1879No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC18760.18760000000000002No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG18140.1814No Hit
TGACCGCCCCCCGGCCGGCGACCGGCCGCCGC18060.1806No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATT17160.1716No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCC17090.1709No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG16630.1663No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT16600.166No Hit
GCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC16490.16490000000000002No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT16440.16440000000000002No Hit
GCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGC16340.1634No Hit
AGGGGCTCGTCCGAAAGCCGCCGTGGCGC15810.1581No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGG15540.1554No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGC15150.1515No Hit
GGCGGGGGACCGCCCCCCGGCCGGCGACCGGC15030.1503No Hit
ACCGCCCCCCGGCCGGCGACCGGCCGCCGC14920.1492No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGC14600.146No Hit
GCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGC14570.1457No Hit
CAGAGTGGCGCAGCGGAAGCGTGCTGGGC14170.14170000000000002No Hit
CCCCGGATAGCTCAGTCGGTAGAGCATCAGAC13880.13879999999999998No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC13860.1386No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC13640.1364No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAT13600.136No Hit
CCCGGATAGCTCAGTCGGTAGAGCATCAGAC13530.1353No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT13000.13No Hit
GGAGGCGTGGGTTCGTATCCCACTTCTGACAC12710.1271No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGC12550.1255No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGC12480.1248No Hit
ACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGC12400.124No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC12240.12240000000000001No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC12190.12190000000000001No Hit
GCGCCGGGTTTCGCCCCCGCGGCGTCGGGC12080.1208No Hit
GCGAGAGGTAGCGGGATCGTTGCCCGCATCCTCCAC12070.1207No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCA11960.1196No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC11910.1191No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC11890.11889999999999999No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATCAGAC11570.1157No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT11500.11499999999999999No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAA10840.1084No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC10830.1083No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAC10730.10729999999999999No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC10540.10540000000000001No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT10430.1043No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC10240.10239999999999999No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCC10210.1021No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCC10080.1008No Hit
GCGAAAGGTCCCCGGTTCGTAACCGGGCGGAAA10010.1001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)