FastQCFastQC Report
Tue 11 Jul 2023
SRR7956050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7956050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75262208
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG21597402.8696208328089448Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA14873431.9762149417673207Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA14149711.8800551267377115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT14040111.8654927051834567No Hit
ACTCTGGACTCTGAATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACG13173111.7502954470854748No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC9033741.200302281857051No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGT8018011.0653434456772781No Hit
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTAGATCGGAAGAGCACACGTC7114820.9453376653525765No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT6292820.8361195036956661No Hit
ACTCGGCTCGTGCGTCGATGAAGAACGCAGATCGGAAGAGCACACGTCTG5930710.7880063789784111Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGAGTCTAACGCGTGCGCGAGTAGATCGGAAGAGCACACGTCTGAACT5468830.7266369331072509Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC5457450.7251248860517088No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG5046200.6704825880208033TruSeq Adapter, Index 11 (100% over 49bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG4611720.6127537475382067Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCT4505890.598692241396904Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT4242500.5636959255832622No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC4140140.550095474211971No Hit
CGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAAC4069580.5407202509923705Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGGAGAGGCCGGGAGGGAGGAAGACAGATCGGAAGAGCACACGTCTG4009510.5327388215875888Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG3992910.5305331993448824No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACAGATCGGAAGAGCACACGTCTGA3751510.49845866865877764Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCT3675860.488407143197287Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACAGATCGGAAGAGCAC3490340.46375732160289534No Hit
AAAGTAAACGCTTCGGGCCCCGCGGGACAGATCGGAAGAGCACACGTCTG3427520.4554105029711592Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC3395290.45112814123125383Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA3360620.4465215795954326Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGA3250100.43183691873616037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGCCAGAGGAAACTCTGGTGGAGGTAGATCGGAAGAGCACACGTCTGA3157960.4195943866010415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTG2976250.39545079517199394Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGA2879600.38260902470466984Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGT2686690.35697730260584437No Hit
AGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCAGATCGGAAGAGCACA2641830.35101680779814487No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA2605290.34616178148799465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGCCCTCCTACTCGTCGCGGCGTAGCAGATCGGAAGAGCACACGTCT2420910.32166343033677675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTCTGAACT2399920.31887451401904127Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGTCCGGGGAGACCCCCCGTCCTTTCCGCAGATCGGAAGAGCACACGTC2307850.30664128270060853No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCT2271600.30182478834530074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT2262110.3005638633402836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGGGCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTC2179890.28963938979839654No Hit
GAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA2146700.2852294740010817Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA2006750.2666344840693486Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC1965120.2611031555173082Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1964670.2610433645528975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG1939410.25768709841730925No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1925210.2558003613181266Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC1895970.251915277319528No Hit
ACTTCCACCGTGGCGGTGCGCCGCAGATCGGAAGAGCACACGTCTGAACT1875060.2491369905065767Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA1837090.24409196179841017Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAA1772160.2354647899779927Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGTCTCGTTCGTTATCGGAATTAACCAGATCGGAAGAGCACACGTCTG1738460.23098711108767897Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTAGCTGCGAGAATTAATGTGAATTGCAGATCGGAAGAGCACACGTCT1656080.22004137853622366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTCT1650930.21935710416574544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCTGGACTTTGAATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACG1595860.21204001880997164No Hit
TCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAA1528980.20315375281044107Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGTGCGGCCGGAAAGGGGGCCGCCCTCTCAGATCGGAAGAGCACACGT1503650.1997881858581667No Hit
ATGGTGACCTCCCGGGAGCGGGGGACCACCAGATCGGAAGAGCACACGTC1487580.19765298408465506No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAAGATCGGAAGAGCACACGTCTGA1435910.19078765268220674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGT1410060.18735299394883553No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1373800.18253517090542973Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCAGATCGGAAGAGCACAC1369390.1819492194542047No Hit
GCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACT1333360.17716195623705328Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCCGGGGAGACCCCCCGTCCTTTCCGCAGATCGGAAGAGCACACGTCTG1313150.17447667759096305Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCT1288120.17115097128162915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCTCAGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTG1285490.17080152631185097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA1282080.17034844367042754Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT1244650.16537516411955386Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGGCGCGACCCGCCCGGGGCGCAGATCGGAAGAGCACACGTCTGAACT1231350.16360800894919267Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1225460.1628254116594613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTCCCCGGACCCCGTCCCGGCCAGATCGGAAGAGCACACGTCTGAAC1215470.16149805224954336Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACGACCTGCTTCTGGGTCGGGGTTTCGTAGATCGGAAGAGCACACGTC1214400.16135588262305567No Hit
ATCTAAGGCTAAATACCGGCACGAGACCAGATCGGAAGAGCACACGTCTG1206190.16026502969458456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGGGGACCGCCCCCCGGCCGGCGACCAGATCGGAAGAGCACACGTCT1200670.15953159386447976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGCCCGACGGCGCGACCCGCCCGGGGCGCAGATCGGAAGAGCACACG1194730.1587423531342583No Hit
GAGCGTACGCGTTAGGACCCGAAAGATAGATCGGAAGAGCACACGTCTGA1185230.15748009944114316Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGTC1173550.15592819174266054No Hit
AGGATTCCCTCAGTAACGGCGAGTGAACAGATCGGAAGAGCACACGTCTG1146020.15227031340882266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1142140.15175478242679247No Hit
GGAGAGGGAGCCTGAGAAACGGCTACCAGATCGGAAGAGCACACGTCTGA1129950.15013511163531104Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCTCCCTCCCGGCTCCGGCCGGGGGTAGATCGGAAGAGCACACGTCTG1119700.1487732063348447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTG1115880.1482656474814026Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTCTGA1098770.14599226214569735Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC1078560.14330698349960713No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGA1070030.14217361255199953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG1062890.14122492925001617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCGCACCGGACCCCGGTCCCGGCGCGCAGATCGGAAGAGCACACGTC1052450.13983777887568752No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1046030.13898476111676128No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACAC1040590.13826195479144063No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCAC1031940.13711263958665684No Hit
CTTCCCCGCGGGGCCCCGTCGTCCCCCAGATCGGAAGAGCACACGTCTGA976560.12975436489984454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCAGATCGGAAGAGCACACG953900.12674355766974044No Hit
AAGCCGGCGGGAGCCCCGGGGAGAGTAGATCGGAAGAGCACACGTCTGAA951390.12641005695713844Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC945590.12563941786028918No Hit
AACCGAGGCTCCGCGGCGCTGCCGTATCAGATCGGAAGAGCACACGTCTG942190.1251876639069638Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACG901100.11972808451221628No Hit
GGCGGGGGACCGCCCCCCGGCCGGCGACAGATCGGAAGAGCACACGTCTG900660.11966962223590358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGGAAAGAAGACCCTGTTAGATCGGAAGAGCACACGTCTGAACTCC900100.11959521570241469Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA883200.11734973281676774Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCT881980.11718763286880982Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTAGATCGGAAG872530.11593202261618474No Hit
GGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTAGATCGGAAGAGCACAC859660.11422200103403822No Hit
GAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCTGAA848020.11267540808794767Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAATCAGTGAAATTGACCTTTCAGTGAGATCGGAAGAGCACACGTCTGA832470.11060929809553288Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTC828330.11005922122295428No Hit
AGGTTCCCCACGAACGTGCGGTGCAGATCGGAAGAGCACACGTCTGAACT815910.1084089906052185Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTC810380.10767422608701567No Hit
AGCTCACGTTCCCTATTAGTGGGTGAACAGATCGGAAGAGCACACGTCTG809360.10753869990101805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCGTCCGAAAGCCGCCGTGGCGCAGATCGGAAGAGCACACGTCT807130.1072424024551605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGGACTTGGGCCCCCCACGAGCGGCAGATCGGAAGAGCACACGTCTG806740.10719058361933788Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAGCCGGCGGGAGCCCCGGGGAGAGTAGATCGGAAGAGCACACGTCTG802310.10660197479191681Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCAGATCGGAAGAGCACACGT796080.10577420210685289No Hit
GGCTCGGGGGGGGCGGCGCGTCTCAGATCGGAAGAGCACACGTCTGAACT782750.10400306087219764Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCGGGGGACCGGCTATCCGAGGCCAACCAGATCGGAAGAGCACACGTC775720.10306899313929244No Hit
GAGACGGGCCGGTGGTGCGCCCTCGGCGGACAGATCGGAAGAGCACACGT773660.10279528339110115No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC769620.10225849339950271No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCAGATCGGAAGAGCACACGTCTG759330.1008912733466443Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACAGATCGGAAGAGCACAC757740.10068001193905976No Hit
GCCGGGTTTCGCCCCCGCGGCGTCAGATCGGAAGAGCACACGTCTGAACT755930.10043951939331888Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)