FastQCFastQC Report
Mon 10 Jul 2023
SRR7956051.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7956051.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82494176
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG36201444.38836312517383TruSeq Adapter, Index 9 (100% over 49bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT26749223.2425586019551247No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG9088251.1016838328077851No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT8549701.036400436316862No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG7150400.866776340671613Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA6764190.8199597023673526Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG5721210.6935289589412954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG5517630.6688508531802293No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGC4824750.5848594693521152No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG4548550.5513783179069514No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA4524130.5484181089341387Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG4213830.5108033323467587Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG4147320.5027409450092574No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA3844920.4660838117832706Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC3746040.45409751107763047No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT3535610.42858904366776146No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT3508860.425346390513677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC3319580.4024017404574112No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC3317390.4021362671711516No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA3216550.389912373935343Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3141300.3807905178663764No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC3015210.36550580249446946No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC2996600.3632498856646559No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG2794200.33871482030440553No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG2714640.3290705031104256No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC2435300.2952087187342777No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA2426150.2940995495245628No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2314310.28054222882352325No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC2240560.2716022037725427No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2227590.2700299715703567No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2139210.2593164879906189No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT2068420.2507352761484641Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2039720.2472562426709978Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1990540.2412946096946286No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1878070.2276609199660349No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1803290.21859603761603727No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGCAGATCGGAAGAGCACACGTC1692060.20511266152897872No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA1640070.1988103984455824No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1637900.19854734957289588No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG1615360.19581503547595894No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC1580290.1915638262754452No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1525080.18487123260677213No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGAGATCGGAAGAGCACACGTCT1452600.17608515781768644Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC1438570.1743844316961236No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1436460.1741286560641566No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC1417880.17187637585470275No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA1394070.16899011149587093No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1327700.16094469505338171No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACAC1296790.15719776387608259No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA1290720.1564619543566324Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC1279890.15514913440677314No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACA1271460.1541272440856916No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGA1216850.14750738282421294Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACG1187770.1439822854888568No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC1107210.1342167476162196No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA1084160.13142261097316738Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCAGATC1073410.1301194886776007No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1034730.12543067282713388TruSeq Adapter, Index 9 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1032200.12512398451013074No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1026660.12445242195036896Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA1015790.12313475317336342Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1009990.1224316732371507No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA984150.11929933090064442Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTGA975910.11830047250850775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCT972970.11794408371325511Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC955310.11580332652816605No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG949210.11506388038835613TruSeq Adapter, Index 9 (100% over 50bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGT908850.11017141379774495No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC904290.10961864750306738No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACAC840120.10183991655362434No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAGATCGGAAGAGCAC834970.10121563005853892No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA832500.10091621498225523Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)