FastQCFastQC Report
Mon 10 Jul 2023
SRR7956053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7956053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52822884
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC614996511.642614969678672No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC11110242.103300531640794No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA10405141.969816718072417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA10308571.9515348688647898No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT8118101.536852853395888No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT7214391.365769805374504Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG7062641.3370417260822034No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT6800741.2874609421174352No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG6599231.2493127031837188No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC6261751.1854237265803207No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG5531811.0472374056668319No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT5428811.0277382810071485No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC5350651.012941663692577No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG4656940.8816141125501591Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG4342390.8220660575821646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG4251770.8049106141194411Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA4104980.7771215218010437No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT3551300.6723033146013004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC3379310.639743562657427No Hit
GTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGA3241350.6136260943268451No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT2901980.5493793182515366No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAATGAGATCGGAAGAGCACACGT2156720.4082927391847821No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT2126910.4026493517468679Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2102480.39802446227661487Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1825760.3456380761035312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1635010.3095268331051368No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1605930.30402164334684945No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACG1585210.3000991009881248No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA1584450.2999552239518009Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1575860.29832903481756123No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG1482010.2805621139504613No Hit
TGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAG1444580.2734761699115103No Hit
AGCTCTTTCTCGATTCCGTGGGTGGTGGTGCAGATCGGAAGAGCACACGT1430610.27083148280960956No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT1383680.2619470758166101No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC1357120.25691895202087034No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1328080.251421334738179No Hit
AGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAGATCGGAAGAGC1251240.23687460911827532No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG1225700.23203958345023343No Hit
GTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGAG1172640.22199469457214793No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTAGATCGGAAGAGCACACGTC1157050.21904332220860942No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1139500.21572089854086726No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC1137700.21538013713904755Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA1137080.21526276376730966No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1125600.2130894632712595No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1119150.2118684015814055No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC1111940.21050346285522767No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACGT1092130.20675319431631184No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCAC1079510.20436407826577588No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACGT1077550.20399302696157218No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGC1072920.20311651291133595No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTCT1023630.19378532985817284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAGC1010910.19137728261864687No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC1009090.19103273497902917No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC980910.18569792592165169No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG968770.18339967957826764TruSeq Adapter, Index 4 (100% over 49bp)
ATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTAGATCGGAAGAGCACA948170.17949985464633095No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGC947570.17938626751239103No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGTC931490.1763421323228016No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA904700.17127046679238492Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACGT880200.16663232548983883No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC860910.1629804991336709No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC847690.16047779594919503Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCACACG828170.15678242785835017No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAGATCGGAAGAGCACACG818690.1549877511420997No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA817500.1547624699931189Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGTC806380.1526573217774327No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACA786060.14881050417466793No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTC775650.14683976740081062No Hit
AAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTCTGAA771400.14603519186873629Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT752350.14242880036614433No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA735310.13920292576225107No Hit
GCTTGGCTGAGGAGCCAATGGGGCGAAGCTAGATCGGAAGAGCACACGTC732170.13860848642796558No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT717570.1358445328354279Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG703810.13323960123040612No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAAGATCGGAAGAGCACACG702990.13308436548068825No Hit
CGTACGCAGTTTTATCCGGTAAAGCGAATGAGATCGGAAGAGCACACGTC697620.13206776063192613No Hit
GTGCGGGCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTC686400.12994368122724992No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG686320.12992853627605794No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAA685960.129860383995694Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGAGATCGGAAGA679680.128671505327123No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATAGATCGGAAGAGCACAC674990.12778363256349276No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACG666270.12613283288356616No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC663410.1255914008784526No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACACGTCTG660390.1250196789709551Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACG658990.12475464232509531No Hit
ATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCAGATCGGAAGAGC643170.12175972822687986No Hit
AAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTCTGA629470.11916615533525204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT629360.11914533102736306No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT626790.11865879947032049No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTCT621320.11762326343256835Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCAC617970.1169890686014039No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT609870.11545564229321519Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT599820.1135530577997218No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC590650.11181706776934026No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT587980.1113116050233077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC574090.10868206287259893No Hit
AGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCAGATCGGAAG571580.10820689002895034No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACAC568790.10767870985612979No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGA566800.1073019791952291No Hit
AGGGGCTCGTCCGAAAGCCGCCGTGGCGCAATAGATCGGAAGAGCACACG566250.1071978576557842No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT563350.10664885317507465Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCACAC557360.10551487495457461No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC548780.10389057893923398No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC538100.1018687279551037No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGATCGGAAGAGCACA537800.10181193438813375No Hit
ACGGTGAAGAGACATGAGAGGTGTAGAATAGATCGGAAGAGCACACGTCT534740.10123264000504023Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCA533830.1010603661852314No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC533230.10094677905129149No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACGTC528690.1000873030711462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)