FastQCFastQC Report
Fri 7 Jul 2023
SRR8070862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8070862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54496339
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC52268789.59124612022103No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT26149274.798353518756553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT25312714.6448459592854485No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC21283963.90557611585615No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT15505242.845189288770389Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC14495762.6599511574529804No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT11570222.123118765831224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC9534931.749645971631232No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT9492941.7419408668901595No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC6494911.191806664297211No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG5446010.9993350195505794No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT5249680.9633087462994532No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG4944330.9072774595005363Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4368180.8015547613207559No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC4026100.7387835722322558No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA2963730.5438402018161257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2886560.5296796175610989Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAG2812990.5161796281397911Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA2744200.5035567618588104No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG2576140.47271799303802775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC2527740.46383666249580546No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2241740.41135607292812826No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACAC2206720.40492995318456165No Hit
GCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2037990.3739682403252813Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2036200.373639777894071Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1874540.34397539988878884No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC1805110.331235094526258No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1797700.32987537016018637No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1725260.3165827341172404No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC1685370.3092629763625039No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG1650340.30283502163328807No Hit
CGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAACTC1628270.2987852083054607Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC1461490.26818131764777814No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT1392500.2555217516538129No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1343860.24659638145600937TruSeq Adapter, Index 6 (100% over 49bp)
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT1335280.24502196376897903Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTG1321590.24250986841519756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1297400.23807103812973565No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG1270780.23318630633151338No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA1234720.22656934808042792No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC1178420.21623837887532224No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT1164520.2136877488229072No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1147400.21054625339144342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA1127100.20682123252352785Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG1091250.20024280897107602No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACAGATCGGAAGAG1090540.20011252498998142No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA1085710.19922622692140846No Hit
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCA1070110.19636364930862601Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA1044130.19159635659195381Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCAGATCGGAAGAGC1035880.19008249343134775No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGT1005250.18446193238778846No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC986270.1809791296255699No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC971010.17817894152486097No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTG942290.17290886274030262Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG888710.16307700963178462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG854490.15679768874015554No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT843970.15486728383717666No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGT843340.15475167974127582No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA823180.15105234867244935Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG743130.1363632885504474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCAGATCGGAAGAGCAC737690.135365056357272No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG730330.13401450691944647No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC726400.13329335755930322No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGGCTCCCTCGCAGATCGGAAGAGC721780.1324455941893638No Hit
AGCCCGACCGACCCAGCCCTTAGAGCCAGATCGGAAGAGCACACGTCTGA691020.12680117833236468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTGAACTCC687860.12622132286721133Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT664810.12199168094576042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCT642330.117866633206315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC639740.11739137192316718Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT638240.11711612407578424Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA631660.11590870351859783Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGCGTCACTAATTAGATGACGAGGCAGATCGGAAGAGCACACGTCTG618930.11357276678714143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTGAAC608440.11164786684111018Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTC603380.11071936410260513No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT599860.11007344915407986No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCT594370.10906604203265836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGA587880.10787513634631493Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC577190.1059135366872993No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT572510.10505476340346459No Hit
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA572380.10503090859002473Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA571350.10484190506815513Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC566400.10393358717179148Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGT560630.10287480045219183No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT553820.10162517522507339Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG552200.10132790754989983Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)