FastQCFastQC Report
Mon 10 Jul 2023
SRR8070864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8070864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62937411
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC53249068.460637187633917No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT29652334.7113997110557975No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT23890183.7958631631669757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC16335262.5954769572583785No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG12199771.938397180017462TruSeq Adapter, Index 12 (100% over 49bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT11603861.8437142258679817No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT11569521.838258011598221No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT9922461.5765599255425362Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG9731781.5462631597604166Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC9112621.4478860593741296No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT7457891.1849693022803243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT6926801.1005854689510506Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC6169010.9801817237127851No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG5493890.8729132502765327Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4602310.7312518781555855No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4233060.6725824803946893No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG4015540.6380211604191981No Hit
GCGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAAC3633990.5773974401330236Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC3230840.5133417388268481No Hit
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT3154610.501229705810428Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA3037070.48255400909325613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2051380.3259396863337769No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT2014280.32004494115590487Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC1837880.2920170961592303No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1705640.2710057456923355No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1600070.2542319384570808Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1524060.24215486080290147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1501270.2385338030507801No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC1496150.2377202964386317No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1471640.23382595130899175No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC1420170.22564798542475795No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTG1243080.1975105077010556Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAACT1240420.19708786559396288Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1188130.1887796115413772No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG1146470.18216033703706053No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGT1143830.18174087269017153No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA1123470.17850591280280023Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1070070.17002129305890895Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC1066630.16947471830387176No Hit
GCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAG1041410.16546756268700027Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACAC1028920.16348305143978675No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTG971780.1544041905378027Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG951030.15110726432645918No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG889660.14135630714139163Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC887890.14107507536336378No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT880340.1398754708864653No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC875360.13908420859574283No Hit
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC873030.13871399953201125Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT866910.13774160490967766No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT858960.13647844522870506No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG850910.13519939674671397No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC848480.13481329888196386No Hit
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAAC841520.13370743833107465Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG815220.1295286836632031Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG802270.12747108393130438No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCT790050.12552947244684087Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTGAACTCC783430.12447763381941465Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA781200.1241233135567016No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC750280.11921049628177428No Hit
CGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAACTC738880.11739917296566267Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA690950.1097836706374846Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC689060.10948337229823452No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG675390.10731137319900243Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC673830.10706350790311346No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT672550.10686013125007637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT671290.10665993235724298No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT655010.10407323555142745No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT652330.10364741568413102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGATCGGAAGAGCACACG647900.10294354179901045No Hit
GCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTGAAC645850.10261782137813073Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGAA645010.10248435544957513Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA643610.10226191223531582Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT643250.10220471255164915No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)