FastQCFastQC Report
Mon 10 Jul 2023
SRR8070866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8070866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57786650
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC28904735.001973639240205No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC27631914.781711692925615No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT15977372.7648894684152827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT10860101.8793441045639434No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT9718671.681819243718056No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT9536811.65034830709169No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT6711661.161455111171871Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT6407951.1088979894145101Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG5714710.9889325648744131No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG3706250.6413678591854692TruSeq Adapter, Index 2 (100% over 49bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3380730.5850365092975627No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC3375040.5840518528068334No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3150140.5451328291222973No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG3125640.5408930955506159No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC3068530.5310101900698517No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC2966200.5133019477682129No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA2826010.48904201922070234No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC2787840.48243668736637263No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG2516090.4354102547906826Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTC2447310.42350785172699923No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACAC2316850.40093170308367077No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC2285370.39548407806993485No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA2169910.3755036846745745Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGA2167270.37504683175093206Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2110470.3652175718786259Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC2075960.3592456043048005No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1995950.3453998458121383No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAAAGATCGGAAGAGCACACGTCTG1963110.3397168723225866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1883420.325926489941881Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCT1811520.31348416978662025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT1791230.3099729781878687No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1634300.28281618678362563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG1607220.2781299833092938No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC1471810.25469723543413575No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTG1458060.2523177931234982Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCA1408670.24377083634368837Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACA1397380.2418170978937177No Hit
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCAGATCGGAAGAGCAC1363000.2358676268653746No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCT1261480.21829955534712603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCAGATCGGAAGAGC1256060.2173616224508602No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT1148920.19882100796637286No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1134540.1963325439353207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACAGATCGGAAGAGCAC1132620.19600028726358076No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1130390.19561438498338282No Hit
ATGCGCGTCACTAATTAGATGACGAGGCAGATCGGAAGAGCACACGTCTG1111370.19232296732895918Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1097820.18997813508829461No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1075370.1860931547338356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAAC1038940.17978893048827022Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCT1025070.177388722135649Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG1022000.17685745756156482No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT1010640.1748916055871036No Hit
GCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT980390.16965683250370112Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT968350.167573306291332Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGT959720.16607988177200098No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTC944450.16343740292956938No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT926610.1603501846879859No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGT918580.1589605903785736No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC903850.15641155872506884No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACAC901040.15592528724194948No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC887670.1536116040642605No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC875690.15153846087288328No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG872110.1509189406203682No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC856330.14818820609950567No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT838550.1451113708789141No Hit
TCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGA790820.13685167767987935Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCGGTCCGGGTTCCTCCCTCGGGGGGCTCCCTCGCAGATCGGAAGAGC784540.13576492148273No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGT774680.13405864503306558No Hit
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCAGATCGGAAGA762200.1318989766667561No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT753960.13047304178387223No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG748310.12949530730713754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACG747540.12936205853774185No Hit
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGA735630.12730102887085512Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGT734300.1270708719055353No Hit
CGGAAGGGCACCACCAGGAGTGGAGCCTGCGGAGATCGGAAGAGCACACG713820.1235268007403094No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC704340.12188628342359352No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA701920.12146750157692131Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA698650.12090162693286426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG682900.11817608392249766No Hit
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTG662750.11468911937272709Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCACGTTCCCTATTAGTGGGTGAACAGATCGGAAGAGCACACGTCTG662740.11468738886922845Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCAC641570.11102391296259605No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC623310.10786401357406944No Hit
TAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACA614210.10628925539030208No Hit
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAAC613630.10618888618738065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT613100.10609716950195243No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA607390.10510905200422588No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAACAGATCGGAAGAGCACACGTCT607140.10506578941675976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT584710.10118427006929802Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC582090.1007308781526529No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)