FastQCFastQC Report
Mon 17 Jul 2023
SRR8070868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8070868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63341136
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC29157414.603234460461839No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG18345492.896299491692097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT16664042.6308400910271015No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT15407142.4324066432910203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG14268562.2526529994662554TruSeq Adapter, Index 4 (100% over 49bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC11796391.8623584521755343No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC9644781.5226724067594872No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT8209871.2961355792545306Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT8202881.295032031001149No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT7946261.2545180749521132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA7100491.120991893798684Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT7053131.113514920224986Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT5968210.9422328642795418No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4953420.782022602183832No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG4853130.7661892896900365Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC4578690.7228619960336676No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA4315270.6812744880357056Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT4127560.651639717986744Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA3588530.5665402022470831Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT3567430.5632090336996798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC3438330.5428273341987425No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC3357280.5300315422192617No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACAC3134370.494839562081741No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC2934860.46334186365081925No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC2623030.4141116130282223No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2572490.4061325960431149No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA2555110.4033887235618887Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG2465970.389315720513759No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG2446020.38616610854595346No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTC2429860.38361484391438766No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTG2267120.3579222197719978Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC2160880.3411495493228918No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2022650.3193264484552345No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCAGATCGGAAGAGCACACGTC1762920.2783215002648516No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG1665440.2629318173264212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA1615330.25502068671455463Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCT1566240.2472705888950271Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCTCGACTGCCGGCGACGGCCGGGTAGATCGGAAGAGCACACGTCTGA1524140.240624039328881Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1508620.23817381488074355No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC1434690.22650209494190315No Hit
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAAC1423410.22472126170897852Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA1417580.2238008487880609Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT1351370.21334792606182498No Hit
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC1344760.21230437041735406Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGA1334830.21073666882134856Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCAGATCGGAAGAGCAC1314370.20750654045737352No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCT1269160.20036899874988032Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT1261770.19920230038185613No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGT1192940.1883357444047104No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1164460.18383945624214887No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGT1153940.18217860822704537No Hit
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCAGATCGGAAGAGC1138660.17976627384769353No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA1138440.17973154128464006No Hit
ATGCGCGTCACTAATTAGATGACGAGGCAGATCGGAAGAGCACACGTCTG1138080.17967470618146159Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1133640.17897373990892743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1117660.17645089282895082No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1113980.17586991177423783Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT977660.1543483527039995No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCT953490.15053250702671325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGAGATCGGAAGAGCACACGT887250.14007484804187914No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC878330.13866660048534651No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT855260.13502441762332776No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC853020.13467077698132854No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGA837590.13223476130898568Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGACCGACCCAGCCCTTAGAGCCAGATCGGAAGAGCACACGTCTGA818300.1291893470303406Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTG797150.12585028471860688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAAC789140.12458570367288645Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCA777560.12275750785397975Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACA751290.11861012407481925No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACA727880.11491426361535416No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG716370.11309711906650995No Hit
AAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAAC712690.11251613801179694Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT684870.1081240475383959Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC684000.107986696039048No Hit
ACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACGTCTGAACT672300.10613955518574847Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGCTAAGACTTCACCAGTCAAAGCGAAAGATCGGAAGAGCACACGTCTG667440.10537228129283945Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG665750.10510547205847397No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA644310.10172062591362427No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGT634150.10011661300169923No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)