FastQCFastQC Report
Fri 7 Jul 2023
SRR8130804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8130804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43452948
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAA2545620.5858336700193506No Hit
CGCCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAA2231750.513601516748645No Hit
CGCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAA2147540.4942219340331063No Hit
CCGTCCTCCCGACCCCTCCACCCGCGCGTCGAAAAAAAAAAAAAAAGATC2099300.48312027068911406No Hit
CCGTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAA2071580.47674095667801414No Hit
GCCTCCTCCCGACCCCTCCACCCGCGCGTCGAAAAAAAAAAAAAAAGATC1966810.45262981927025986No Hit
GGCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAA1839350.4232969417863202No Hit
CCGAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAAAA1786560.41114816881929395No Hit
GCCCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAA1767660.4067986365389985No Hit
GGCCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAA1540990.3546341665932539No Hit
CCGCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAA1513480.34830318071860167No Hit
CGCTCCTCCCGACCCCTCCACCCGCGCGTCGAAAAAAAAAAAAAAAGATC1474430.3393164486791552No Hit
CCCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAA1366100.3143860342916205No Hit
GGGAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAAAA1324430.30479635121649284No Hit
GGCTCCTCCCGACCCCTCCACCCGCGCGTCGAAAAAAAAAAAAAAAGATC1284030.29549893829988244No Hit
CCCCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAA1251760.28807251466574835No Hit
GCGTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAA1136410.261526559716961No Hit
CGGAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAAAA1133410.26083615776770774No Hit
GCGTCCTCCCGACCCCTCCACCCGCGCGTCGAAAAAAAAAAAAAAAGATC1080520.24866437140237296No Hit
CGCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAAAA1078660.24823632219383596No Hit
CCCTCCTCCCGACCCCTCCACCCGCGCGTCGAAAAAAAAAAAAAAAGATC1036620.23856148954496714No Hit
CGGTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAA936730.21557340597466484No Hit
GCGAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAAAA888380.2044464278925333No Hit
CGGTCCTCCCGACCCCTCCACCCGCGCGTCGAAAAAAAAAAAAAAAGATC838290.19291901667983494No Hit
GGGTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAA827270.19038294018624466No Hit
GGCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAAAA789880.18177823055871836No Hit
CCCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAAGA778620.17918692190918786No Hit
GCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAAAA754130.1735509406634505No Hit
GGGTCCTCCCGACCCCTCCACCCGCGCGTCGAAAAAAAAAAAAAAAGATC717050.16501757257068034No Hit
GCGCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAA685590.15777755746284464No Hit
GCCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGCAAAAAAAAAAAAAAA662200.15239472359850015No Hit
GCCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGAAAAAAAAAAAAAAAG633450.1457783715848232No Hit
CGGCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAA625470.14394190239980956No Hit
CCGTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGAAAAAAAAAAAAAAAG618450.14232636183855696No Hit
CGCCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAAG610490.140494495333205No Hit
CGCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGCAAAAAAAAAAAAAAA559500.12875996353573063No Hit
CCGTCCTCCCGACCCCTCCACCCGCGCGTCAAAAAAAAAAAAAAAGATCG553140.12729631140331377No Hit
CGCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGAAAAAAAAAAAAAAAG549290.12641029556843875No Hit
CCGTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGCAAAAAAAAAAAAAAA546700.12581424855225012No Hit
CCCCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAAG540220.1243229803418631No Hit
CCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAAAA518980.11943493454115012No Hit
GCCCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAAG518180.11925082735468259No Hit
GGGCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAAAAAAA517150.11901378935210563No Hit
GCCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAAGA498890.1148115428209842No Hit
CCCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGAAAAAAAAAAAAAAAGAT495350.11399686852086538No Hit
GGCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGCAAAAAAAAAAAAAAA485040.11162418715526504No Hit
GCCTCCTCCCGACCCCTCCACCCGCGCGTCAAAAAAAAAAAAAAAGATCG464550.10690874184186537No Hit
GGCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGAAAAAAAAAAAAAAAG461420.10618842247481115No Hit
GCCTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTAAAAAAAAAAAAAAG439520.10114848824526244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)