FastQCFastQC Report
Thu 28 Sept 2023
SRR8130830.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8130830.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28125701
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG12663634.502511777395344TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC6118422.1753840019845194Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG2968701.0555114697407897Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGTCGATCAGATC1812220.6443288293507778Illumina DpnII expression Sequencing Primer (95% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1784610.6345121851362923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1128590.4012664431012759Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG842080.29939875987446496TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCAGACGATCAGATC666280.23689365111290916Illumina DpnII expression Sequencing Primer (95% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC647200.23010982019612594No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA547470.19465114842826495Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA503530.17902842670481353Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC490490.17439209781829082No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC487620.17337167880722332No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC469990.16710339059638016No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG453660.16129731308741424No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT443030.15751785173283325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG431590.15345039755631334No Hit
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC413400.1469830031969692Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA390380.13879831830680417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT365340.12989542909526058No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATGTCGTATG356270.12667062058293233TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCAGTCGATCAGATC332780.11831882874670395Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGGCGATCAGATC330980.11767884469795081Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATG330800.1176148462930755TruSeq Adapter, Index 1 (97% over 49bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGAGATCGGAAGAGCACACGT327710.11651620700938263No Hit
AAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCTGAACT285240.10141613892574623Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)