FastQCFastQC Report
Thu 28 Sept 2023
SRR8130831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8130831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38906075
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5197131.335814522539218No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC3501670.9000316788573506No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1986440.5105732202490227No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1723680.4430362096407823TruSeq Adapter, Index 2 (100% over 49bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1382560.3553583855477583Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1142450.2936430878725238No Hit
CGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA1044220.26839510282134604TruSeq Adapter, Index 2 (100% over 36bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1039330.2671382296980613No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC990810.2546671695872688No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT946750.24334246001427798No Hit
CCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG765710.19680988123320073TruSeq Adapter, Index 2 (100% over 35bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT679240.17458456038035192Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGCTCCTACACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA621190.15966401134013133TruSeq Adapter, Index 2 (100% over 36bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC618670.15901629758334657No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT595680.15310719469902836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC594090.1526985181620094Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC567070.14575358732537272Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG552380.14197782737014722No Hit
CGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC534510.13738471434088378Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC530590.13637715960810745Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC481150.12366963257023486Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG458900.11795073134465504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC440080.1131134405102545Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC418730.10762586562638354Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA407540.10474970811113687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCTGAACT396470.10190439410811808Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)