FastQCFastQC Report
Thu 28 Sept 2023
SRR8130834.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8130834.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38536992
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11085252.876521862422474Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7379581.9149340976067877Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC7066751.833757549110216Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG6864131.781179496313568No Hit
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT5582661.4486496507044453Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT3591920.9320706712137783No Hit
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2734360.7095416269126558Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2397090.622023120019331TruSeq Adapter, Index 4 (100% over 49bp)
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1767730.4587099065749605TruSeq Adapter, Index 4 (100% over 36bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1075660.2791240167369576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT1041490.2702572115644106Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT986010.2558606546146622Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC915390.23753540494286632Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC844020.21901553707149743Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT735670.19089969450651467Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC722240.18741473127949373Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA635430.1648883234062482Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC633980.16451206155374037No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT619330.1607105193887473No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC593150.153917046768985No Hit
GCCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC560350.14540574417432475Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA499570.12963388528092695Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT451220.11708749868178606Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACATTCCGACGATCAGATC442610.11485328175068776Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG441820.11464828391380417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG426380.11064174391192753TruSeq Adapter, Index 4 (100% over 35bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT419930.10896802739559952No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA419070.10874486519342247Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG417970.10845942516738204No Hit
TGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT414650.10759791527060546Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCCTCCCACGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA407210.10566730273084107Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGTCGGGGCGGCGGCGGCGGTAGATCGGAAGAGCACACGTCTGAACTC403460.10469421173297594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)