FastQCFastQC Report
Thu 28 Sept 2023
SRR8130835.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8130835.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29592255
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC14615834.939072740485644Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG12003364.056250529065798TruSeq Adapter, Index 6 (100% over 49bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCTACGATCAGATC5047201.70558140973035Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCAGACGATCAGATC4174851.4107914385030813Illumina DpnII expression Sequencing Primer (95% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG3707731.2529393248334741Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCATACGATCAGATC2528260.8543654412277807Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1821240.615444818247207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1373520.46414847398415565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA1076840.3638925117399806Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC766990.25918606067702515No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG684310.23124631765980658No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT657850.22230478887127728No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCTTCGATCAGATC656560.22186886399836714Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGATCGGAGGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC570340.19273286202758122Illumina DpnII expression Sequencing Primer (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA565720.1911716427152983Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTACAGAGTCCTACAGTCCGACGATCAGATC514710.1739340242911532Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACGGTCCGACGATCAGATC507380.17145702481950092Illumina DpnII expression Sequencing Primer (95% over 23bp)
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC496310.16771618114266723Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTACGGAGTTCTACAGTCCGACGATCAGATC470710.15906526893607803Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACGCGTCTACAGAGTTCTACAGTCCGACGATCAGATC461980.15611517270312789Illumina DpnII expression Sequencing Primer (96% over 25bp)
AGGTCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC450070.1520904709695155Illumina DpnII expression Sequencing Primer (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA446290.15081310971401132Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCATTCGATCAGATC444680.1502690484385188Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGATCGGAAGAGCACACGTCTACAGAGCTCTACAGTCCGACGATCAGATC436690.14756901763654037Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGGGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC418680.14148296572870164Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGACCGACGATCAGATC387240.13085856417498432Illumina DpnII expression Sequencing Primer (95% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC386970.13076732408530545No Hit
AGATCGGAAGAGCGCACGTCTACAGAGTTCTACAGTCCGACGATCAGATC369690.12492795834585774Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGATCGGGAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC369260.12478265005488767Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGCCCGACGATCAGATC353560.1194772078031904Illumina DpnII expression Sequencing Primer (95% over 23bp)
GGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC348590.11779771430058304Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC342170.11562822772377435No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGTCGATCAGATC337510.11405349136116866Illumina DpnII expression Sequencing Primer (95% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA325470.10998485921400719Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGGTCAGATC318320.10756868646880746Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGGCGATCAGATC318100.10749434269203208Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACACGTCTACAGAGTACTACAGTCCGACGATCAGATC310430.10490244829263604Illumina DpnII expression Sequencing Primer (95% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT309240.10450031604553285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGCCTACAGAGTTCTACAGTCCGACGATCAGATC300900.10168201105322998Illumina DpnII expression Sequencing Primer (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)