FastQCFastQC Report
Thu 28 Sept 2023
SRR8130836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8130836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43199078
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC8930072.0671899525263018No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA5996051.3880041606443545Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG4746911.0988452114649299Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG3202860.7414186015729317TruSeq Adapter, Index 11 (100% over 49bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG3109220.719742213016676Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC2895890.6703592146110156Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2499430.5785841077441514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA2299330.5322636747015758Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT2198790.508990029833507Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA1994060.4615978146570628Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1803190.4174140012895645Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1513830.3504310902191014Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1478570.34226887897931524Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1399710.3240138597402472No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1319580.3054648527452368No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC1305910.30230043335647117No Hit
CCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1282910.296976245650428Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC1195540.276751276960124Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1140430.26399406024360056No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA952420.22047229804302765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG842720.19507823754942177No Hit
CGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAAC816150.1889276433168319Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG780380.18064737400182476TruSeq Adapter, Index 11 (100% over 35bp)
CACTCGCCGAATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG756280.17506855123157952TruSeq Adapter, Index 11 (100% over 35bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT731000.16921657448337207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG727520.16841100173480555Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG727240.16834618553664502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG698480.16168863603987102TruSeq Adapter, Index 11 (100% over 35bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG696310.16118631050412696No Hit
CGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC674070.1560380524788052TruSeq Adapter, Index 11 (100% over 36bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG651980.15092451741678375No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCAGACGATCAGATC611320.14151227949818743Illumina DpnII expression Sequencing Primer (95% over 23bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG587240.13593808645638222Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC559220.12945183691188963Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT549840.12728049427351204Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC548020.1268591889854686Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC543030.12570407173967926Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC529230.12250955911605335Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTGAACTCCAG529100.12247946588119311Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC503270.11650017160088463No Hit
CCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC501720.1161413676467817Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT468940.10855324273356019No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC468100.1083587941390786No Hit
ACTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG460010.10648606898508343TruSeq Adapter, Index 11 (100% over 35bp)
ACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA442390.10240727822941037Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)