FastQCFastQC Report
Thu 28 Sept 2023
SRR8130838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8130838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43761050
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG12801012.9252063193182067Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA8415391.923032011343421Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT5580841.275298467472787Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5102401.1659683668467735No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA4308440.9845376196412107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG3888530.8885824266099648Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC3388860.7744009798667993Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA3137250.7169046446554641Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC2961740.6767982029681646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA2680850.6126109862537577Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT1887690.431363050018224Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1327170.3032765438672061TruSeq Adapter, Index 5 (100% over 49bp)
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC1248550.28531079578757823Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA1051280.24023189571548217Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC975090.2228214359573182Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG909240.20777380798678277No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTACGACGATCAGATC894760.20446492942925273Illumina DpnII expression Sequencing Primer (95% over 23bp)
CCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC883570.2019078609859681TruSeq Adapter, Index 5 (100% over 35bp)
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA776620.1774683194301782Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA759770.1736178633739364TruSeq Adapter, Index 5 (100% over 36bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC703190.1606885575186153No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGTCGATCAGATC686520.1568792339306301Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAACT665980.15218556227512822Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC664350.15181308492369358No Hit
CGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTC619800.1416327990301878Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGAA609260.139224264500052Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAAC584450.1335548392920188Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA558970.12773230989658613No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC558800.12769346256545488Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT555560.1269530781368363No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT460790.10529683359974225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG453050.1035281374647089No Hit
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA444910.10166803584466094Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)