FastQCFastQC Report
Thu 28 Sept 2023
SRR8130839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8130839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41105307
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA7684211.869396085522485Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG5401701.3141125548581842Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC4797781.1671923530458002No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT4671551.1364834229312533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG4055730.9866682178045769Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT3559050.8658371046833442Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC2251080.5476373160283172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2155980.5245016172729229No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA2010440.4890949969063606Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1288750.31352399338606085No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1277330.31074576331469805No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1265230.3078021044825185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTC1226110.29828508518376956Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA1176680.2862598739379322Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1136850.2765701275506834Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA1094160.2661846072576468Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC747950.18195947301889753Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG736120.17908149913586582Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG717910.1746514142322304Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG695240.16913631127970896Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC685820.1668446363872188No Hit
CGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC668460.16262133743460425Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA665700.16194989128776Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC660380.16065565451195876Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT658520.16020315819560718Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT651070.1583907401543066No Hit
CCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC649440.15799419768352538Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCGAGGGCGCACCACCGGCCCGTAGATCGGAAGAGCACACGTCTGAAC598530.1456089356053222Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC596320.14507129213266792Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAAC576370.1402179042234133Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG525510.12784480602468193Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC453310.11028016406737942Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA425990.10363382032397908TruSeq Adapter, Index 9 (100% over 35bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA413280.10054176216224343Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)