FastQCFastQC Report
Thu 28 Sept 2023
SRR8130840.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8130840.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37305308
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA9080522.434109376606675Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG7141251.914271824266938Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC6799071.8225476117232433TruSeq Adapter, Index 5 (100% over 35bp)
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC5579941.49574961289691Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT3946831.057980810666407Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT3808381.0208681295433883TruSeq Adapter, Index 8 (100% over 36bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC3363470.9016062808005767Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA3175260.8511550152594907Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT3122870.8371114373321888Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2011410.5391752830455119No Hit
CGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAACTC1919660.5145809277328577Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1864860.49989132913739787Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC1729980.46373561639003225Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAAC1723530.4620066399130119Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA1561990.4187044910606287Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1552550.4161740200617028TruSeq Adapter, Index 8 (100% over 49bp)
CGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACT1527270.40939750450525697Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1493280.4002862005589124TruSeq Adapter, Index 5 (100% over 35bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC1366940.3664197062787955Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1314410.3523386001798993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTGAACTCCAG1247230.3343304389820344Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1241070.3326791994318878Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1199150.32144219262309803Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAACTCC984760.263973158993889Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCAGACGATCAGATC962000.2578721505261396Illumina DpnII expression Sequencing Primer (95% over 23bp)
GCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAACT824740.2210784588616719Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA775330.20783369487259026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC673050.18041668493931212No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG672130.18017007124026427Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA658840.1766075755224967Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC634590.17010716008563714No Hit
CGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA627180.1681208475748277Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT549510.14730075409108Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC536260.14374898070805367No Hit
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC531910.14258292680494689TruSeq Adapter, Index 5 (100% over 35bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA521720.13985141202962323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCA517830.1388086649760404Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT511100.13700463215583156No Hit
CGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACACGTCTGAACTCCA476400.12770300676783045Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA469350.12581319526969192Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCGAGGGCGCACCACCGGCCCGTAGATCGGAAGAGCACACGTCTGAAC455140.12200408585287649Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA436810.11709057595771626Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTAAGACGATCAGATC436040.11688417101394795Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGGGACGGCTGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC421210.11290886540864373TruSeq Adapter, Index 5 (100% over 35bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTTTGACGATCAGATC388480.10413531500664733Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)