FastQCFastQC Report
Fri 29 Sept 2023
SRR8130841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8130841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7158401
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG166230223.22169434207444TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC2997334.187150175018136Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGACCGACGATCAGATC482120.673502364564377Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATATCGTATG435710.60866945006294TruSeq Adapter, Index 10 (97% over 49bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA416970.5824904192989467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC348330.4866030835657293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCTACGATCAGATC238490.3331609950322705Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAGCTCGTATG214480.29961998496591624TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATG198350.2770870198526179TruSeq Adapter, Index 10 (97% over 49bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA198200.2768774758497044Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC181330.2533107603220328No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT176600.24670313943016045Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGTCGATCAGATC175900.24572526741656414Illumina DpnII expression Sequencing Primer (95% over 23bp)
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC160250.22386284311258897Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG159220.22242397429258293Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA158430.22132037587723852Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC137320.19183054986721196Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG134980.18856166342176137TruSeq Adapter, Index 10 (97% over 49bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG132670.18533468577689347TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACATTCCGACGATCAGATC129380.18073868731299073Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGACCTACGATCAGATC114240.15958871261892146Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGATCGGAAGAGCACACGTCTACAGAGTCCTACAGTCCGACGATCAGATC107500.1501732020880082Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAGGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC105640.14757485645188081Illumina DpnII expression Sequencing Primer (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG101470.1417495331708855Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACGGTCCGACGATCAGATC99760.13936073153767162Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACACGTCTACGGAGTTCTACAGTCCGACGATCAGATC93610.13076942741821812Illumina DpnII expression Sequencing Primer (95% over 23bp)
CGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT90990.12710939216732897Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACGCGTCTGAACTCCAGTCACTAGCTTATCTCGTATG90950.12705351376655205TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACGCGTCTACAGAGTTCTACAGTCCGACGATCAGATC90880.1269557265651924Illumina DpnII expression Sequencing Primer (96% over 25bp)
AGATCGGAAGAGCACACGTCTACAGAGCTCTACAGTCCGACGATCAGATC89970.12568449294751718Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGGTCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC89450.12495807373741705Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC86870.12135391688730486TruSeq Adapter, Index 10 (100% over 50bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG85990.12012459207021234TruSeq Adapter, Index 10 (97% over 49bp)
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA84210.1176380032356388TruSeq Adapter, Index 10 (100% over 35bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTGCGACGATCAGATC83750.11699540162670406Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACTAGCTTATCTCGTATGC83340.1164226480187405Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG81440.11376842398183618TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGGACTCCAGTCACTAGCTTATCTCGTATG80960.11309788317251297TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGGGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC79670.11129580474745686Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGATCGGGAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG79050.11042968953541439TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTGAACTCCA78780.11005251033017009Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAGCTCCAGTCACTAGCTTATCTCGTATG78190.10922830391871033TruSeq Adapter, Index 10 (97% over 49bp)
CCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC75510.10548445106665581Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAGGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG74270.10375222064257086TruSeq Adapter, Index 10 (97% over 49bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC73520.10270450062800339Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCAGATCGGAAGAGCACACGTCTGAACTCCAG73000.10197808141790324Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCGCACGTCTACAGAGTTCTACAGTCCGACGATCAGATC71660.10010615499187597Illumina DpnII expression Sequencing Primer (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)