FastQCFastQC Report
Fri 7 Jul 2023
SRR8135336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21205460
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT7663383.613871144507122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC5273612.4869113898024375Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC4889202.305632605942055Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA2953021.3925753084347146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC2848001.3430503276043055TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2831371.3352080077489477TruSeq Adapter, Index 9 (100% over 49bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA2779471.310733179096327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC2290321.0800614558703279Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT2145271.0116592613411828Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT1668150.7866606053346638Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG1587660.7487033999734031Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA1545760.7289443379205167Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA1362480.642513767680588Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT1340940.6323560064247604No Hit
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC1222480.5764930352843088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA1196550.564265052491198Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC1084550.5114484665741748No Hit
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC1027620.4846016073218879Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGAA1020840.4814043175672681Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC985290.4646397673052129Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG949010.4475309660813771Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACTCAAAGGACCTGGCGGTGCAGATCGGAAGAGCACACGTCTGAACTC940570.44355085907120145Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG822620.38792839202733637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA809460.38172244318208615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACTCAAAGGACCTGGCGGTGCAGATCGGAAGAGCACACGTCTGAACT771520.3638308247026945Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGA768940.36261415691996307Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA723610.34123758692336786Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA698600.3294434546574325Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA693920.32723647588875693Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTCC681600.3214266514378844Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA638550.3011252762260286Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA625290.29487216971478103Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTC621190.29293870540889Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA613890.289496195791084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC596480.2812860461409467Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGA588890.2777067792917485Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC575220.27126032634991176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG571610.2695579346074077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG538690.25403363096108267Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA520090.24526230508557703Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA515820.2432486727474905Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAACT500170.23586849801890647Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA466730.22009897450939522Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAA460420.2171233257849629Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTCAAAGGACCTGGCGGTGTAGATCGGAAGAGCACACGTCTGAACTC455400.21475601095189636Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT447240.2109079454065132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAA444010.20938475279479907Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAAC433320.2043435982996832Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGAA407410.19212504703977185Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT394760.18615960229110803No Hit
AAACTCAAAGGACCTGGCGGTGCTAGATCGGAAGAGCACACGTCTGAACT386080.18206631688253874Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCCTCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAA381940.1801139895102488Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCC372000.17542651751011296Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAACTCAAAGGACCTGGCGGTGTAGATCGGAAGAGCACACGTCTGAACT367050.1730922130432445Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACT356510.16812179504712466Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA343770.16211390839906326Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA336650.1587562825800525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA335520.15822340095428253Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAAATAGGAGAGATCGGAAGAGCACACGTCTGAACTC330660.1559315383868117Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT330100.15566745545722657No Hit
AACTTAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACT328390.15486105936867203Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACAAGATCGGAAGAGCACACGT322430.15205046247523044No Hit
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG319530.1506828901613075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACTCAAAGGACCTGGCGGTGCTAGATCGGAAGAGCACACGTCTGAAC316240.14913140294999494Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGAGATCGGAAGAGCACACGTCTGAACTCC315250.14866454205662127Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC310530.1464387002215467No Hit
AATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT308740.1455945780001943Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC300590.1417512282214109No Hit
ATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAACT292920.13813423523941476Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTCGCGCCGTCGGGCCCGGGGGAGGAGATCGGAAGAGCACACGTCTGAA287040.13536136447877103Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC285000.13439934809242526Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT281040.1325319045189305Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCTGGGCTGTAGTGCGCTATGCCAGATCGGAAGAGCACACGTCTGAACT277430.13082951277642646Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAAC275530.12993351712247694Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCT264560.1247603211625685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGGCTTTAACATATCTGAACACATAGATCGGAAGAGCACACGTCTGAA263630.12432175486879324Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACGTCTGAA261040.12310037131946205Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC255240.1203652266916162Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGAA250670.11821012135553768Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAACT249570.11769138702956693Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCTG247650.11678595984241794Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA237390.11194758331109064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCA237100.11181082607969835Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGAACT233090.10991980367320493Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG232290.10954254234522619Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC230360.10863239939147748Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGTTGGGGCGACCTCGGAGCAGAGATCGGAAGAGCACACGTCTGAACT227000.10704790181396678Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGCGCGCGCCGCGGCTGGACGAGAGATCGGAAGAGCACACGTCTGAAC225150.10617548499301595Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC224450.10584538133103455Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTGGGCTGTAGTGCGCTATGCCGAGATCGGAAGAGCACACGTCTGAAC217150.10240287171322858Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCAAGACGAGAAGACCCTATGGAGCAGATCGGAAGAGCACACGTCTGAA216180.10194544235305435Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACT215860.10179453782186286Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGACGAAAAATAACAATACAGGACTAGATCGGAAGAGCACACGTCTGA214060.1009456998339107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph