FastQCFastQC Report
Fri 7 Jul 2023
SRR8135337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16980756
Total Bases849 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT6179473.6391018161971114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC4009492.361196403740799Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC3773122.221997654285828Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA2311641.3613292600164564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC2216251.3051539048084786TruSeq Adapter, Index 9 (100% over 50bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA2185981.2873278433539708Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1906291.1226178622435892TruSeq Adapter, Index 9 (100% over 49bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1720891.0134354442169713Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT1674400.9860573934399622Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA1405480.827689886127567Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT1307820.7701777235359839Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG1234990.7272879958937046Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT1140540.6716662085009643No Hit
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA1068520.629253491422879Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC958530.5644801680207877Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA930040.5477023520036446Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC863800.5086934880873384No Hit
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC837800.4933820378786433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGAA778100.4582245925917551Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC767640.45206467839241077Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTCAAAGGACCTGGCGGTGCAGATCGGAAGAGCACACGTCTGAACTC760140.4476479139091334Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG716810.42213079323441194Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG700630.41260235998915473Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACTCAAAGGACCTGGCGGTGCAGATCGGAAGAGCACACGTCTGAACT599970.35332349160426074Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA598700.3525755861517591Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGA592430.34888317104373917Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA565710.33314771144464944Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA544260.32051576502247603Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA524700.3089968432500885Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTCC512090.3015707898988714Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC484050.2850579797507249Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA483920.2849814224996814Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA482560.28418051587338045Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTC480210.28279659633528687Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA473620.27891573260931374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG454270.267520480242458Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGA436250.25690846744397006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG431910.2543526330629803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC423780.24956486036310754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA411490.24232725562984359Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA403050.23735692333132868Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAACT399190.2350837618772686Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAA372430.21932474620093476Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA365050.21497864994938976Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCCTCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAA358790.21129212386068086Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT352660.20768215502301549Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTCAAAGGACCTGGCGGTGTAGATCGGAAGAGCACACGTCTGAACTC352610.2076527099264603Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAA351240.20684591428084828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGAA334140.19677569125897573Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAAC332290.19568622268643399Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT310110.1826243778545549No Hit
CCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACT297640.1752807707736923Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTCAAAGGACCTGGCGGTGCTAGATCGGAAGAGCACACGTCTGAACT293050.17257771090992652Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAACTCAAAGGACCTGGCGGTGTAGATCGGAAGAGCACACGTCTGAACT280840.16538721833115086Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCC280760.1653401061766626Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA273460.16104112207960586Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACAAGATCGGAAGAGCACACGT269700.15882685081865613No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT263860.15538766354101077No Hit
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA261740.15413919144707103Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAAATAGGAGAGATCGGAAGAGCACACGTCTGAACTC251210.14793805411254954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA247600.14581211814126532Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC246820.14535277463500448No Hit
GGTCGCGCCGTCGGGCCCGGGGGAGGAGATCGGAAGAGCACACGTCTGAA246310.14505243465014162Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTTAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACT241470.1422021493035999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT241400.14216092616842266Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAACTCAAAGGACCTGGCGGTGCTAGATCGGAAGAGCACACGTCTGAAC238840.14065333722479728Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGAGATCGGAAGAGCACACGTCTGAACTCC238030.14017632666060334Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG236230.13911630318461676Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC235400.13862751458180073No Hit
ATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAACT227140.13376318463088452Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC221310.1303298863725502Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAAC219250.12911674839447665Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT212810.12532421995816911Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCTGGGCTGTAGTGCGCTATGCCAGATCGGAAGAGCACACGTCTGAACT210480.12395207845869759Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGAA209240.12322184006412908Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCT205820.12120779545975455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACGTCTGAA204170.12023610727343353Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGGCTTTAACATATCTGAACACATAGATCGGAAGAGCACACGTCTGAA202150.11904652537260414Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC202060.11899352419880481Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCTG193390.11388774445613611Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG193370.11387596641751403Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAACT193150.11374640799267123Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA187320.1103131097343369Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGAGATCGGAAGAGCACACGTCTGAAC186310.10971831878392223Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGAACT180290.1061731291586782Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCA178320.10501299235440402Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGAGATCGGAAGAGCACACGTCTG177130.10431219905639066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC172900.1018211438878222Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGTTGGGGCGACCTCGGAGCAGAGATCGGAAGAGCACACGTCTGAACT170660.10050200356215Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGGGCTGTAGTGCGCTATGCCGAGATCGGAAGAGCACACGTCTGAAC170120.10018399651935403Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)