FastQCFastQC Report
Fri 7 Jul 2023
SRR8135338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27161532
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT11743014.3233975167527365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC7156782.634895557437629No Hit
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA6037382.222768583156502Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG5996332.207655297204885TruSeq Adapter, Index 10 (100% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC5882452.1657283543505574TruSeq Adapter, Index 10 (100% over 50bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC4124041.518338509035499Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA3622001.3335035740988395Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT3397821.2509677289189727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA2939331.0821664992976094Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA2919601.074902549679451Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA2600310.9573502702277618Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT2573110.9473361075509289No Hit
AGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGA2344500.8631692792586221Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA2136520.7865977515553982Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC2055560.7567908908820018No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG2014840.7417991002863903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT2005520.7383677768985932No Hit
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1700740.6261576114336997Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC1660660.6114014481951902Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA1501300.5527302362768051Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT1322000.4867177595137122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1268040.46685142796805423No Hit
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA1191670.43873445724637333Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA1175940.4329431786101019Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA1173510.4320485309886055Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG1127260.41502077276053506Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG1071750.3945837812093957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC1052180.3873787384305127Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC1046790.3853943142824197Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA1039620.38275455154738697Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA964570.3551235622497288Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG910480.3352093688971594Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG890770.32795278263391037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGACGAAAAATAACAATACAGGACTAGATCGGAAGAGCACACGTCTGA873350.32153930050779167Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAA857920.3158584721951619Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC856400.31529885722204476Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA780610.28739542379273747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAAC771960.28421077279440643Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACG752490.2770425467900706No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT684330.2519482332587131Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA646580.23804990086715286Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACAAGATCGGAAGAGCACACGT638950.2352407809692031No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC638600.23511192225828792No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT634790.2337092031480404No Hit
AGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAACT576240.21215298165066682Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGA573830.2112656973840798Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG561230.2066267837911352Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT552800.20352312969680797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA538760.198354054550384Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAA532810.1961634564648268Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTC529740.1950331814862284Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC514120.18928240130195895No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCAGATCGGAAGAGCACACGTCT493010.1815103801950494Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTG464400.17097710099710134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT464360.17096237428728248Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACAC458350.16874968613699698No Hit
AATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT437450.16105498025663648Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGAA436690.1607751727700779Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGAA436300.1606315873493439Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG432060.1590705561085435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACGTCTGAA428300.15768624538556958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTC404430.1488980813011578No Hit
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC401800.14792980013056703Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGGATTTAGCAGTAAACTGAGAGCAGATCGGAAGAGCACACGTCTGA397020.14616995830721183Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGA393120.14473410409987184Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC381720.14053699180149337No Hit
AAAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTG381670.14051858341421977Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC362600.13349762450807268No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC361200.13298218966441214No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTG349880.12881453078567143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACG346110.1274265383852428No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGATCGGAAGAGCACACGT342940.1262594466320972No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGTAGATCGGAAGAGCACACGTCTG340460.12534639062332714Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGTGGATTTAGCAGTAAACTGAGAGCAGATCGGAAGAGCACACGTCTG336820.1240062600298098Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGT335710.12359759383233611No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGT317410.11686012409020227No Hit
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG311410.11465111761737151Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACACG306350.11278818882528423No Hit
ATCGGTCGTCCGACCTGGGTATAAGGGAGATCGGAAGAGCACACGTCTGA301530.11101362029211019Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG300830.11075590287027992Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAAC300410.11060127241718178Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAACTCAAAGGACCTGGCGGTGCAGATCGGAAGAGCACACGTCTGAACT299380.11022205963934581Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGAAGATCGGAAGAGCACACGTCTGAACTCCAG295810.10890770078801151Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGGTGGATTTAGCAGTAAACTGAGAAAGATCGGAAGAGCACACGTCTGA295460.1087788420770964Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCTG294940.10858739484945105Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA288970.10638943340898445Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGTCGCGCGCGCGTCCGCTGGGGGCGAGATCGGAAGAGCACACGTCT278390.10249421866189284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)