FastQCFastQC Report
Fri 7 Jul 2023
SRR8135339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16323567
Total Bases816.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT7065514.328410573497814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC4282672.623611616260098No Hit
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA3549552.1744940918856765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC3243631.9870840729847832TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3017011.8482541223986155TruSeq Adapter, Index 10 (100% over 49bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC2387811.4627991541309568Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA2155081.3202261491008676Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT2015621.2347913908767612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA1730201.0599399016158662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA1728451.0588678320124516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT1622650.9940535668460208No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA1515250.9282591237564681Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGA1363430.8352524910762459Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA1245400.7629459909099525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC1225270.750614127414676No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT1198340.7341165077461317No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG1170070.7167979890669729Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC981340.6011798769227339Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG953330.5840206371560823Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA919610.5633633874262899No Hit
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA882630.5407090251781367Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT794840.4869278877588459Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA705940.43246675190538925Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA692470.4242148790151074Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG672620.41205454665637725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG671950.41164409715107Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA668940.40980013743319704Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC612620.37529787453930874Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA607930.37242472800215787Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC601670.3685897818779437Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA560690.3434849748219859Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG548330.3359131003658698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG527940.323421957958086Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC513050.3143001771610335Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAA505460.3096504581382243Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGACGAAAAATAACAATACAGGACTAGATCGGAAGAGCACACGTCTGA500290.3064832582241369Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACG477940.29279139786052893No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA446490.2735247755591655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAAC436090.2671536190588736Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT413660.2534127497991095Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACAAGATCGGAAGAGCACACGT402500.24657600878533475No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT387270.23724594017961884No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC383410.23488126094008743No Hit
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA380510.23310468845442908Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGA340800.20877789762494925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAACT340510.20860024037638344Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG334330.20481430314832536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA326580.200066566333204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT324090.19854116444034567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAA319060.19545973009453144Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTC313850.19226802573236598Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC313160.19184532400301968No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACAC288620.17681184510713865No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCAGATCGGAAGAGCACACGTCT275640.16886015170581284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTG273860.1677697037663398Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT262780.16098197164872113Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT260890.15982413647703347Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGAA260250.15943206530778475Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACGTCTGAA251830.15427387898735614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTC250430.15341622330462454No Hit
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG247350.15152938080261502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGAA246860.15122920131365897Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC243590.14922596268327873No Hit
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC243160.14896253986643973Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGGATTTAGCAGTAAACTGAGAGCAGATCGGAAGAGCACACGTCTGA230120.14097408979299683Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGATCGGAAGAGCACACGT227410.13931391343570923No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC222410.13625085742595353No Hit
AAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGA220840.13528905783889023Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTG218200.13367176426573923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACG216580.13267933411857838No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC213260.13064546492810059No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTG210940.12922420693957393Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGTAGATCGGAAGAGCACACGTCTG210930.12921808082755443Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGT203360.12458061402878427No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGCAGATCGGAAGAGCACACGTCTG201510.12344728330517467Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG195300.11964296774105806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACACG195180.11956945439682394No Hit
TCTCGTCGCGCGCGCGTCCGCTGGGGGCGAGATCGGAAGAGCACACGTCT192780.11809918751214118Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGT192090.1176764857827949No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG180750.11072947475266894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAAGGGAGATCGGAAGAGCACACGTCTGA179380.10989019740599587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAAC178910.10960227014107884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCTG173710.10641669189093292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA172220.10550390120002572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACTCAAAGGACCTGGCGGTGCAGATCGGAAGAGCACACGTCTGAACT171390.10499543390240626Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGTGGATTTAGCAGTAAACTGAGAAAGATCGGAAGAGCACACGTCTGA168520.10323723975280649Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT166260.1018527384363969Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)