FastQCFastQC Report
Fri 7 Jul 2023
SRR8135340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21125504
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT18121558.578043865841023Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA11250235.325425608780742TruSeq Adapter, Index 15 (97% over 40bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA5985912.833499262313458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT5699622.697980602024927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC3728471.7649141057179039No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCG2961881.402039922929176TruSeq Adapter, Index 15 (97% over 36bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC2868151.3576717506952733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG1612710.7633948046872633Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT1610700.7624433480971625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC1402780.6640220275928091Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA1394500.6601025944753791Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG1362160.6447940839659968Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG1304620.617556863968784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC1201040.5685260810818998Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC1175460.5564174942287767No Hit
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA1173680.5555749107808268Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT1105050.5230881118859934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT1086550.5143309243651655No Hit
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA1077810.5101937449634337Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC823840.38997412795453307Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAG785400.3717781123707155Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA783630.3709402625376417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT644440.305053077076883No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG560580.26535698272571395Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGCGGCGGCGGCGGCGGCGGCGGGAGATCGGAAGAGCACACGTCTGAAC559320.26476054725132236Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGTCGTCCGACCTGGGTATAGGGGTAGATCGGAAGAGCACACGTCTG544480.2577358627751556Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA536550.25398210617838984Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTACTTGGATAACTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA534120.25283183776349194Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC516880.24467108571705554No Hit
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA516390.24443913858812552Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG505460.23926529752852288Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA498380.23591389819622766Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC447750.21194760607841592Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACGTCTGAACT443650.21000682397920545Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC415010.19644975097398862Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGTCTGAACTC375990.1779791857273559Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCC372020.17609994062153497Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACTTGGATAACTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT362400.17154620311070448Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGAAC349100.16525049532546063Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC344790.16321030731385155Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA344400.16302569633368275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTCCGTAGCGGTCCTGACGTGCAAGATCGGAAGAGCACACGTCTGAAC342760.16224938349399853Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT333900.1580554007137534Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGTCGTCCGACCTGGGTATAAGGGAGATCGGAAGAGCACACGTCTGA332790.1575299694625037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT330450.15642230358149087Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA322740.1527726865119999Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACAAGATCGGAAGAGCACACGT294460.13938602364232353No Hit
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG286810.13576480826208928Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTACCATCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAAC283910.13439205994801354Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCT280350.1327068930521137Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA278130.13165603054961433Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAAC276930.13108799676447955Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGCGCCCCCTCGATGCTAAGATCGGAAGAGCACACGTCTGAACTCC276540.13090338578431077Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGTCGTCCGACCTGGGTATAGGGAGATCGGAAGAGCACACGTCTGAA271870.12869278763716122Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGA270510.12804901601400848Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTG270240.12792120841235316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCA266520.1261603036784353Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGAACT262890.12444200147840259Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGTC261510.12378876262549761No Hit
AGACCCTGTTGAGCTTGACTCAGATCGGAAGAGCACACGTCTGAACTCCA252280.11941963609483588Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAACT244870.1159120274716286Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCCAGATCGGAAGAGCACACGTCT243650.11533452645674158Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC240420.11380556885175379Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA239060.11316179722860104Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCTG235950.1116896430021267Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTCGCGCCGTCGGGCCCGGGGGAGGAGATCGGAAGAGCACACGTCTGAA235260.11136302357567422Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA235070.11127308489302788Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGGCTTGGCGGAATCAGCGGGGAAGATCGGAAGAGCACACGTCTGAAC230790.10924709772604714Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTCCGTAGCGGTCCTGACGTGTAGATCGGAAGAGCACACGTCTGAACT230780.10924236411117103Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCATTCGTAGACGACCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG229500.10863646140702725Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA226290.10711697103179171Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGCGGCGTCCGGTGAGCTCTCGAGATCGGAAGAGCACACGTCTGAAC225560.10677141714583473Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGGGCTTGGCGGAATCAGCGGGGAAGATCGGAAGAGCACACGTCTGAA223630.10585782947474295Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTC223060.10558801342680393Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTC222000.10508625024993486No Hit
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG217540.10297505801518392Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCATTCGTAGACGACCTGCAGATCGGAAGAGCACACGTCTGAACTCCA214980.10176325260689639Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTACCATCTGTGGGATTATGACTTAGATCGGAAGAGCACACGTCTGAA214770.1016638466944978Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)