FastQCFastQC Report
Fri 7 Jul 2023
SRR8135341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22795732
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT19607968.601592613915622Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA10237084.4907880124226764TruSeq Adapter, Index 15 (97% over 40bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA6379392.798501930098143Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT6058842.6578835020520506Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC4014591.7611147560429294No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC2964651.3005285375350089Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCG2914471.278515644946168TruSeq Adapter, Index 15 (97% over 36bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG1838230.8063921790271967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT1735880.7614934234180328Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC1477040.6479458523200746Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA1469360.6445768006046044Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG1456570.6389661011982418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG1408070.6176901886721602Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC1269010.5566875413344918Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA1242780.5451810014260564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC1240230.5440623709736542No Hit
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT1233920.5412943089522196No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT1195730.5245411728827133Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA1172570.5143813763032483Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC855280.3751930405217959Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA851100.37335936393707386Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAG830370.36426555637695684Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT700210.30716714865747674No Hit
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA625750.2745031394473316Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG624710.27404691369419504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGCGGCGGCGGCGGCGGCGGCGGGAGATCGGAAGAGCACACGTCTGAAC598580.2625842416466381Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGTCGTCCGACCTGGGTATAGGGGTAGATCGGAAGAGCACACGTCTG592770.26003551892959614Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA577690.25342024550911546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTACTTGGATAACTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA566260.24840614901070077Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA564220.24751124464877902Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG562730.24685761352168908Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC558650.24506780479784548No Hit
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC487390.21380756713581298Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACGTCTGAACT459680.2016517828863754Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC443370.19449693477708896Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGTCTGAACTC410070.1798889371045422Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGAAC398950.1750108309748509Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCC383650.1682990482604375Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACTTGGATAACTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT374740.1643904218561615Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGTCCGTAGCGGTCCTGACGTGCAAGATCGGAAGAGCACACGTCTGAAC371850.1631226406767723Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT363660.1595298628708216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGTCGTCCGACCTGGGTATAAGGGAGATCGGAAGAGCACACGTCTGA360160.15799448774007346Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC359800.1578365634409108Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA354240.15539751037606514Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT339460.14891384053822004Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA332540.14587818456542656Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACAAGATCGGAAGAGCACACGT329760.1446586580330037No Hit
AGCTACCATCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAAC308110.1351612661528044Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCT306190.13431900322393683Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG304410.1335381553002992Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA298210.13081834792583102Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAAC298120.13077886685104034Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGAACT297800.1306384896962291Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTG296990.1302831600231131Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGAGATCGGAAGAGCACACGTCTGAA290230.12731769262772522Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGGCGCCCCCTCGATGCTAAGATCGGAAGAGCACACGTCTGAACTCC288340.1264885900571212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGTC282770.12404515020618771No Hit
AAGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGA282080.12374246196612594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCA275270.12075506064029881Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAACT267560.117372848566565Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTCGCGCCGTCGGGCCCGGGGGAGGAGATCGGAAGAGCACACGTCTGAA266640.11696926424648263Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGGCGGCGTCCGGTGAGCTCTCGAGATCGGAAGAGCACACGTCTGAAC265160.11632001990548055Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCCAGATCGGAAGAGCACACGTCT261000.11449511689293417Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACCCTGTTGAGCTTGACTCAGATCGGAAGAGCACACGTCTGAACTCCA260330.11420120222504809Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC259890.1140081836371826Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA256630.11257809137254289Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
NTCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT255530.1120955449028792Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCTG255140.11192446024545295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGCTTGGCGGAATCAGCGGGGAAGATCGGAAGAGCACACGTCTGAAC253080.11102078231135547Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTCCGTAGCGGTCCTGACGTGTAGATCGGAAGAGCACACGTCTGAACT250870.1100513025859402Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA249350.10938451110058672Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGGCTTGGCGGAATCAGCGGGGAAGATCGGAAGAGCACACGTCTGAA243610.10686649588615975Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCATTCGTAGACGACCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG240890.10567329007026405Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTC240050.10530480003888448No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA234600.1029140016210052Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTC233280.10233494585740875Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG228880.10040475997875392Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)