FastQCFastQC Report
Fri 7 Jul 2023
SRR8135342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20893068
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG15781977.5536871846681395TruSeq Adapter, Index 16 (97% over 49bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT13317836.374281651694237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT5226512.5015521894630313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA3915451.8740426250467381Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC2335481.1178252997597098Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTC2133681.021238240357998TruSeq Adapter, Index 16 (98% over 50bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC1911150.9147292298096191No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT1463730.7005816474631682Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG1413080.6763391570831052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC1372000.656677133296077Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT1276910.6111644302311178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC1250730.5986339584018967Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA1089090.5212685853508925Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC1052580.5037938899160238Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA1038810.4972031872006543Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG1025330.4907512865032555Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG881490.4219054855897659Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT843880.4039042997419048No Hit
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA818620.39181416534900476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAG785020.3757322763703253Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC745490.35681212543796825No Hit
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA574590.2750146603648636Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTGGGCTGTAGTGCGCTATGCTAGATCGGAAGAGCACACGTCTGAACT574260.2748567132409659Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG555380.2658202232434222Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGCGGCGGCGGCGGCGGCGGCGGGAGATCGGAAGAGCACACGTCTGAAC550240.26336007713180276Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC541240.2590524282982279Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC536620.25684116856365946Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTGGGCTGTAGTGCGCTATGCCAGATCGGAAGAGCACACGTCTGAACT529690.25352427896180685Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGTCGTCCGACCTGGGTATAGGGGTAGATCGGAAGAGCACACGTCTG476660.22814265477908746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA459550.21995333571881354Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACGTCTGAACT411090.19675903988825386Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGGCGCCCCCTCGATGCTAAGATCGGAAGAGCACACGTCTGAACTCC397100.19006303909028582Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA357270.17099929986347626No Hit
TCCTACTTGGATAACTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA352610.16876889502298084Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG347710.16642361954692342Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC346070.16563867020391643No Hit
AACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT345290.16526534063833997Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA343900.16460004820737673Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGTCCGTAGCGGTCCTGACGTGCAAGATCGGAAGAGCACACGTCTGAAC325690.15588423873411028Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCCAGATCGGAAGAGCACACGTCT324910.1555109091685338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA318730.15255299030281239Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCT318220.15230889020224314Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGTCTGAACTC314550.150552326733441Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT297320.14230557235538602No Hit
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACAAGATCGGAAGAGCACACGT291270.13940987508392735No Hit
AGGTCCGTAGCGGTCCTGACGTGTAGATCGGAAGAGCACACGTCTGAACT283930.13589674814632297Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTACCATCTGTGGGATTATGACTTAGATCGGAAGAGCACACGTCTGAA282940.13542290677462976Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTC280380.13419761999530178Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA276110.13215387993759461Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC269780.12912416692464698Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGCCTCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAA268270.12840143917590274Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCA265850.1272431602673193Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTCC261270.12505104563867786Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCTG259810.12435224927234238Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA259670.12428524140159788Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA257020.12301687813393418Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC241450.1155646456518497Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCC239600.11467918450272599Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGAA228820.10951957845539967Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGA225730.10804061902253896Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTACCATCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAAC223210.10683447734913801Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC220840.1057001298229633No Hit
TTCTGGGCTGTAGTGCGCTATGCAAGATCGGAAGAGCACACGTCTGAACT220390.10548474738128455Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCGTATTGCGCCGCTAGAGGTGAGATCGGAAGAGCACACGTCTGAACT219480.1050491962214453Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGGGATAACTGGCTTGTGGCGGCTAGATCGGAAGAGCACACGTCTGAA219270.10494868441532858Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGAAC216110.10343622104709563Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTACTTGGATAACTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT213520.10219657543832242Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG213250.10206734597331517Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAAC211370.10116752599474621Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)