FastQCFastQC Report
Fri 7 Jul 2023
SRR8135343_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135343_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21076832
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG13586356.446106321860895TruSeq Adapter, Index 16 (97% over 49bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT13540716.424452213691318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT5224322.47870268169334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA3966491.8819194459584818Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC2249151.0671195747064832Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTC2003000.9503325736998806TruSeq Adapter, Index 16 (98% over 50bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC1948120.9242945049806347No Hit
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG1524010.7230735624784598Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT1490410.7071318877523909Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC1346550.638876848285359Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT1302430.6179439111152947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC1205420.5719170698898203Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA1090530.5174069803279734Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG1054840.5004736954775746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA1033580.4903867905764965Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC1012260.48027141839912185Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT901380.4276638918030945No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG886520.4206134963736486Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA809360.3840045790562832Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAG756180.35877308316543965Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC746020.3539526243792236No Hit
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA685630.32530031078674443Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG587500.27874208040373427Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGGGCTGTAGTGCGCTATGCTAGATCGGAAGAGCACACGTCTGAACT565690.2683942254699378Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC562520.26689020437226996Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGCGGCGGCGGCGGCGGCGGCGGGAGATCGGAAGAGCACACGTCTGAAC541350.2568460003856367Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC534490.253591241795731Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTGGGCTGTAGTGCGCTATGCCAGATCGGAAGAGCACACGTCTGAACT515410.24453864793342758Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGTCGTCCGACCTGGGTATAGGGGTAGATCGGAAGAGCACACGTCTG489140.23207472546158742Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA470110.22304585432953114Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA433270.20556694668344844No Hit
AGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACGTCTGAACT404760.192040245896537Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGGCGCCCCCTCGATGCTAAGATCGGAAGAGCACACGTCTGAACTCC376390.17857996875431753Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG357040.16939927214867967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA349300.16572699350642447Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC344330.16336895411986013No Hit
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA341650.16209741577861417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA337690.16021857554304175Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGTCTGAACTC331850.1574477606501774Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT331010.15704921878202568Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCT330230.1566791441901705Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCCAGATCGGAAGAGCACACGTCT329660.1564087050653533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTCCGTAGCGGTCCTGACGTGCAAGATCGGAAGAGCACACGTCTGAAC324290.15386088383681193Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCCTCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAA322500.1530116100939648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACAAGATCGGAAGAGCACACGT314300.14912108233343607No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT303600.14404441806055102No Hit
AGGTCCGTAGCGGTCCTGACGTGTAGATCGGAAGAGCACACGTCTGAACT303310.14390682622511772Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTACCATCTGTGGGATTATGACTTAGATCGGAAGAGCACACGTCTGAA282030.13381043223194075Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA279860.13278086573921546Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTC275100.13052246181968905Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGGTGCGGACCAGGGGAATCCGACTAAGATCGGAAGAGCACACGTCTG264170.12533667298766724Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC259330.12304031269974539Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA257820.12232388624628218Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCA253130.12009869414910174Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTCC249880.11855671668303852Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA247120.11724722197339714Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC245410.11643590459894543No Hit
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC239640.11369830152842704Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGAA237920.1128822396079259Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGAAC235190.1115869785364328Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCC233940.11099391028025463Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCGTATTGCGCCGCTAGAGGTGAGATCGGAAGAGCACACGTCTGAACT222450.10554242686946502Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGA222190.10541906867217997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGGGCTGTAGTGCGCTATGCAAGATCGGAAGAGCACACGTCTGAACT220340.10454132765303628Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTACCATCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAAC220270.10450811583069029Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGGGATAACTGGCTTGTGGCGGCTAGATCGGAAGAGCACACGTCTGAA219780.10427563307426847Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAACT218720.10377271119302939Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGCGGCGTCCGGTGAGCTCTCGAGATCGGAAGAGCACACGTCTGAAC218080.10346906024586618Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTC212690.10091174992522595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)