FastQCFastQC Report
Fri 7 Jul 2023
SRR8135344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28250433
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC15570225.511497823767869TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG14452825.115964063276481TruSeq Adapter, Index 11 (100% over 49bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT12058554.268447849985167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA9678803.426071380923613Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC6091392.1562111986035757Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC5533311.9586637840205847No Hit
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA4534111.6049700901929538Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA4094841.4494786681676701Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA3522891.2470215943238816Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT3334081.1801872204932222Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC2337650.8274740426102496Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC2275620.8055168570336604Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA1951890.6909239231837614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA1790360.6337460385120469Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGA1733830.6137357257497611Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA1614590.571527523135663Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT1548860.5482606231203607No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC1505800.5330183788687416No Hit
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1492640.5283600431894265Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAA1360790.48168819217744385Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT1355230.4797200807506207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA1312410.4645627909490803Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA1151020.40743446303991165Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG1081880.38296050187974107Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG1073710.3800685108083122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG1032490.36547758400729646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT1020800.36133959433471335No Hit
ATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGAA985790.3489468639294838Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA953940.33767270045029046Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC946980.3352090213980083Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAAC902710.3195384651272425Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG874850.3096766693806074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC856370.30313517672454793Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG837660.296512269387163Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA794500.28123462744801114Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA770580.27276750059016797Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA769790.2724878588586589Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGA760120.2690649024742382Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTC740530.26213049548656475Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGACGAAAAATAACAATACAGGACTAGATCGGAAGAGCACACGTCTGA708800.2508988092324107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAACT703040.24885990243052203Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT674870.23888837385253528Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT658700.23316456777848324Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT593050.20992598591320707Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAACT586850.20773132928617413Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGAA578720.20485349728975835Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA561300.19868722012154647Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT551300.19514745136826753No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC543240.1922943977531247No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA541990.19185192665896483No Hit
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC540120.19118998990210168Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGA496600.17578491628783177Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAA476570.16869475947501406Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCAAGACGAGAAGACCCTATGGAGCAGATCGGAAGAGCACACGTCTGAA457410.16191256254373163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA448530.15876924789081995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCC431710.15281535684780478Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAAC420900.14898886682551024Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTG419310.1484260435937389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG418170.1480225099558651Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT385770.13655365919524137Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC383820.13586340428835197No Hit
TTAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAAC378750.13406874153043957Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACGTCTGAA365500.12937854793234496Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGAATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA359060.12709893685523332Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGATGTAGATCGGAAGAGCACACGTCTGAA353190.1250210925970586Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAAC340530.12053974535540747Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG317280.11230978300403396Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACG314890.11146377827200028No Hit
AATGGATTTTTGGAGCAGGGAGATAAGATCGGAAGAGCACACGTCTGAAC312130.1104868020960953Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA304250.1076974643185115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTC300300.10629925566096633No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGTAGATCGGAAGAGCACACGTCTG296730.10503555821604574Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGGATTTAGCAGTAAACTGAGAGCAGATCGGAAGAGCACACGTCTGA292960.10370106539605958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG287200.10166215859417094Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAAGGGAGATCGGAAGAGCACACGTCTGA287040.10160552229411847Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCTG282630.10004448427392246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)