FastQCFastQC Report
Fri 7 Jul 2023
SRR8135345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13212460
Total Bases660.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC6810635.15470245510677TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG5763664.362291352253857TruSeq Adapter, Index 11 (100% over 49bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT5737394.342408605210537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA4494173.4014634670606383Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC2804842.1228749226109294Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC2603961.9708366193729252No Hit
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA2124121.607664280535192Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA1910961.4463317202095598Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA1652131.2504333031093378Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT1572231.189960083133648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC1122870.8498568775231865Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC1061360.8033023373391481Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA909700.6885167485842909Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA853280.6458146325513947Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGA789280.5973755076647347Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT736670.5575570332852474No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA735290.5565125646548789Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC708040.5358880935117305No Hit
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG669010.5063477959441315Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAA646490.48930327887463804Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT634890.4805236874889309Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA608760.4607469010312992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA522750.3956492583515863Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG514690.38954895606117257Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT510890.3866728830210271No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG506730.3835243399033942Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG493770.3737154171138456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGAA477700.36155265559933575Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA442170.3346613726739759Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC428380.3242242549835534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG417210.31577011396817856Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAAC410490.3106840058550792Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC400070.3027975108344699Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG392590.2971361881133415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA370410.2803490038948084Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGA359930.2724170971946178Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA357720.2707444336633753Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA356050.26948047524836405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTC345120.26120798095131414Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAACT328560.24867435738689087Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGACGAAAAATAACAATACAGGACTAGATCGGAAGAGCACACGTCTGA325960.24670651793837028Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT320850.24283895656070104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA314530.23805559297814335No Hit
ATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT299670.226808633668522Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT277350.2099154888642993Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAACT272720.20641122092328Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA269980.20433742088906987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT266440.2016581317937765No Hit
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC262900.1989788426984831Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGAA256890.1944301061270952Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC252400.19103179877176543No Hit
AAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAA223450.16912066337381534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGA222450.16836380204746126Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAAGACGAGAAGACCCTATGGAGCAGATCGGAAGAGCACACGTCTGAA217810.1648519654931784Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAAC208720.15797209603661996Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG199640.1510997951933251Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA198810.1504716002924512Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCC197110.14918493603764932Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTG187160.1416541658404264Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC183720.1390505628777684No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT180810.13684809641807807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAAC178100.1347970022236586Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACGTCTGAA171240.1296049335248697Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGAATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA166770.12622176339606703Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGATGTAGATCGGAAGAGCACACGTCTGAA165820.12550274513603069Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAAC161880.12252071151019567Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACG154690.11707887857370997No Hit
AATGGATTTTTGGAGCAGGGAGATAAGATCGGAAGAGCACACGTCTGAAC147480.11162190841069718Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTC146080.11056230255380149No Hit
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA143560.10865501201138925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGTAGATCGGAAGAGCACACGTCTG142880.10814034630946849Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG141540.10712615213215404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG141380.10700505431993738Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCTG136730.10348564915239099Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGGATTTAGCAGTAAACTGAGAGCAGATCGGAAGAGCACACGTCTGA134620.10188867175378394Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAAGGGAGATCGGAAGAGCACACGTCTGA132340.10016302792969667Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)