FastQCFastQC Report
Fri 7 Jul 2023
SRR8135346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20225155
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT10907775.3931700399823885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG7796223.8547145868597794TruSeq Adapter, Index 12 (100% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC5661672.7993209446355296TruSeq Adapter, Index 12 (100% over 50bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC5473112.706090509565934Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA5226522.5841680817773707Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC3614351.7870567617405158Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC3365551.6640416352804217No Hit
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA3309741.6364472855708645Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT3072361.5190785929699921Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA1507830.7455220985945472Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC1505400.7443206244896516Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC1499500.7414034651403165Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA1399530.6919749193516688Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA1290670.6381508571874974Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT1261620.6237875556454326No Hit
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1174810.5808657585071659Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA1050290.5192988632225563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA1025870.5072247901190374Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT1007060.4979244905663269Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC983350.4862014654523043Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA975470.48230532720268404Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC936340.46295813307734845No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT846080.4183305393704029No Hit
AAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAA838520.4145926199329498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA824790.4078040440233956Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG802260.39666445077924Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA788090.38965832400295575Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG766320.3788945004376975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG750830.37123572106122305Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC689640.34098131757210265Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGA684570.3384745382668266Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA657910.32529293347813654Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTC649870.32131768582243253Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT635350.3141385072203402Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGAA608230.3007294628891596Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA576150.2848680269693854Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT485010.23980533152897962Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCAAGACGAGAAGACCCTATGGAGCAGATCGGAAGAGCACACGTCTGAA451630.22330113168477572Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAAC437890.2165076114373413Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTCC435100.21512814116875745Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA433900.21453482062312995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC433790.21448043290644747Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC427850.2115434962055915No Hit
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCC421350.20832967658344276Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAAC405330.20040884729931613Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA398480.19702197585135936Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT392160.19389715431105473Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAACT390740.19319505833206221Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT376840.18632242867854412No Hit
ATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAACT373300.1845721330689431Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA362540.17925202550981686Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAA359060.1775313959274972Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGACGAAAAATAACAATACAGGACTAGATCGGAAGAGCACACGTCTGA338070.16715323071689686Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG335500.16588253588167803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGAA334420.16534854739061333Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA332840.16456734200553716Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGA316830.1566514570592908Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACG314460.15547964898167652No Hit
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG305960.15127696178348202Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC302990.14980849343305405No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA302610.14962060859360532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACAAGATCGGAAGAGCACACGT292930.14483448952554379No Hit
AAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGA283760.14030053168937395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGTAGATCGGAAGAGCACACGTCTG266960.13199404405058948Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAAC266820.13192482332026628Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT257800.12746503055229985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAAGACGAGAAGACCCTATGGAGCTAGATCGGAAGAGCACACGTCTGA256160.1266541591399423Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG250580.12389521860277461Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA249450.12333650842230877Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC243250.12027101893656686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACGTCTGAA241010.11916348725139561Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA234940.1161622741580967Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTCAAAGGACCTGGCGGTGCAGATCGGAAGAGCACACGTCTGAACTC231860.1146394180909862Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGGTCGTCCGACCTGGGTATAAGGGAGATCGGAAGAGCACACGTCTGA228430.1129435101980677Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG226840.11215736047511132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACT225140.11131682303547241Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAACTCAAAGGACCTGGCGGTGCAGATCGGAAGAGCACACGTCTGAACT217370.10747507250253459Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG207220.1024565695541023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAGCAGGGAGATAAGATCGGAAGAGCACACGTCTGAAC205860.10178413960239119Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGAATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA204720.10122048508404509Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)