FastQCFastQC Report
Fri 7 Jul 2023
SRR8135347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22530044
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT12314745.4659191966069836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG7334403.255386451974972TruSeq Adapter, Index 12 (100% over 49bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC5866462.6038386786994288Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC5856352.599351337263256TruSeq Adapter, Index 12 (100% over 50bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA5724712.540922689720446Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC3859651.7131124999134488Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC3772391.6743819941052933No Hit
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA3751331.6650344757427014Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT3388491.5039872980274693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA1684460.7476505594041449Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAAC1674870.7433940208905051Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC1654820.7344947928197566Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA1543520.6850940903621848Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT1497560.664694662824449No Hit
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA1404290.6232966078539394Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1217770.5405093749484022Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA1144470.507975039906713Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT1122780.4983478949264369Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA1090880.4841890233325776Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAA1074860.47707851791146083Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1057860.4695330377517239Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC1034920.45935107805382003No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT958940.42562722025753696No Hit
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG944900.41939554134914253Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAA938390.4165060662997374Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA887990.3941359368849879Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA861020.3821652545374523Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG858100.38086920735707397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG843970.37459758178901026Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC749820.33280893725729077Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGA741120.3289474268226018Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT705230.3130175866500749Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTC698840.310181373813562Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA694610.3083038808091098Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGAA683860.30353247423751145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA651510.2891738693453062Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCAAGACGAGAAGACCCTATGGAGCAGATCGGAAGAGCACACGTCTGAA518030.22992853453814824Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT501030.22238305437841133Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC486050.2157341548023608Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA482030.21394987067047005Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC473670.2102392698389759No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAAC465460.20659524677359709Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAAAGATCGGAAGAGCACACGTCTGAACTCC458750.2036170013693715Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCC452200.20070977224900224Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAAC449750.19962233540245194Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA437390.1941363274745491Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA434950.1930533291457398Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGGATTTAGCAGTAAACTGAGAGATCGGAAGAGCACACGTCTGAACT434290.19276038697483236Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT430320.19099829543164673Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT429580.19066984511881113No Hit
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA403400.17904980567281628Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAACT402730.17875242498416782Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAA395710.17563658552997055Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG383240.17010175390691648Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACG365970.16243643376817196No Hit
AGTGACGAAAAATAACAATACAGGACTAGATCGGAAGAGCACACGTCTGA363290.16124691101357813Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGAA352640.15651988961939Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACAAGATCGGAAGAGCACACGT351290.15592068972435205No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC349020.15491314619714014No Hit
AAGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGA332660.14765173117282862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG328560.1458319388990097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA321300.14260957501902793Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGA315160.13988432512604057Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACGTCTGAAC313400.13910314600362078Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGTCGTCCGACCTGGGTATAGGGGTAGATCGGAAGAGCACACGTCTG298730.1325918404775419Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAAGACGAGAAGACCCTATGGAGCTAGATCGGAAGAGCACACGTCTGA288380.12799797461558443Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT279410.12401662420188793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA276330.12264956073765325Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG270120.11989324122047874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACGTCTGAA263410.11691499581625317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA261210.11593852191322838Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC261130.11590301377130022Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACTCAAAGGACCTGGCGGTGCAGATCGGAAGAGCACACGTCTGAACTC258990.11495317097472157Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCTG256460.11383022598624307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAAGGGAGATCGGAAGAGCACACGTCTGA253030.11230781440107263Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACT246530.1094227778694085Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAACTCAAAGGACCTGGCGGTGCAGATCGGAAGAGCACACGTCTGAACT239780.10642677839421885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG237880.10558346002342471Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAGCAGGGAGATAAGATCGGAAGAGCACACGTCTGAAC226800.10066558236637266Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)