FastQCFastQC Report
Tue 22 Aug 2023
SRR8135942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20297140
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGTTCTCGGGAAATAGTTTCAATCCCCGGTCCCCATCACGGGCGGGG293240.14447355637296683No Hit
ACGACTTTTACTTCCTCTAAACGACCGAGTTCGATCGTCTTCTCGGCGCT272790.13439824527002325No Hit
ACGACTTTTACTTCCTCTAGAGGATCAAGTTTGATCGTCTTCTCGGCGCT265820.1309642639307804No Hit
CTCGTTGATGGGAAACAGTTTCAAGCCCCAGTCCCAATCACGAGCGGGGT254580.12542653792603295No Hit
GGCAGACATTCGAATGAACCGTCGCCGCCGCGAGGGCGCGCGATCGGCCC238800.11765204358840703No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA236640.11658785424941641No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN234920.11574044422022019No Hit
CTCGTTCTCGGGAAATAGTTTCAATCCCCGGTCCCCATCACGGGCGGGGT233070.1148289857585847No Hit
GTCGTTTCGGCCCCAAGGCCTCTAGTCATTCGCTTTACCGGATAAAACTG232980.11478464453612677No Hit
GCATCGTTTACGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC232390.11449396318890248No Hit
CCGGAACCCAAAGACTCGTGGTTTCCCGCACGCTGCCCGGCGGGTCATGG229900.11326718936756607No Hit
GTCGGCATCGTTTACGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA214710.10578337637716446No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGCA209510.10322143907959447No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGGTCCT208940.10294061133736084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)