FastQCFastQC Report
Tue 22 Aug 2023
SRR8135943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8135943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20225686
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGTTCTCGGGAAATAGTTTCAATCCCCGGTCCCCATCACGGGCGGGG298870.147767546673077No Hit
ACGACTTTTACTTCCTCTAAACGACCGAGTTCGATCGTCTTCTCGGCGCT276260.1365886922203776No Hit
ACGACTTTTACTTCCTCTAGAGGATCAAGTTTGATCGTCTTCTCGGCGCT267340.13217845861940108No Hit
CTCGTTGATGGGAAACAGTTTCAAGCCCCAGTCCCAATCACGAGCGGGGT257390.1272589715869217No Hit
CTCGTTCTCGGGAAATAGTTTCAATCCCCGGTCCCCATCACGGGCGGGGT242260.11977838477270931No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA240370.11884392944694189No Hit
GGCAGACATTCGAATGAACCGTCGCCGCCGCGAGGGCGCGCGATCGGCCC239400.11836434126387604No Hit
GTCGTTTCGGCCCCAAGGCCTCTAGTCATTCGCTTTACCGGATAAAACTG235750.11655970531728813No Hit
GCATCGTTTACGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC233530.11546209112511684No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN232540.11497261452590532No Hit
CCGGAACCCAAAGACTCGTGGTTTCCCGCACGCTGCCCGGCGGGTCATGG230130.11378106038035002No Hit
GTCGGCATCGTTTACGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA214670.1061373146997338No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGCA212840.10523252462240343No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGGTCCT212000.10481721114428454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)