FastQCFastQC Report
Mon 17 Jul 2023
SRR8136737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8136737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134027493
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT56786914.236959800479145No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG23821471.7773569785417085No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG20226761.509150066695644No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA19717041.4711190636088374No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA12798210.9548943812595226No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA12716030.94876280346451No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA12703470.9478256823023617No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG12018890.8967481022718228No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT11853430.8844028739685521No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG10633450.7933782660547115No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG8482510.6328932825744938No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT8123970.6061420547499161No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC8049040.6005514107467488No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG8001630.5970140768058685No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA7977210.5951920625718188No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT7809020.5826431447165843No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG7691580.5738807634042666No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT7618470.5684259124357418No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC7489570.5588084826745211No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6348800.4736938562299304No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG6192060.461999240708024No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA6135990.45781577067922924No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG6085650.4540598248748859No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT5669390.4230020179516452No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA5460110.40738731119890453No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT5395760.40258605747404375No Hit
CTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG5256320.39218222189681634No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA4889520.3648147026073225No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG4750470.3544399655375185No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG4651800.34707804315939866No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAAT4502130.33591093134898825No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC4383230.32703961716272645No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATCA4189180.31256124443064826No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA4133500.3084068729092769No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC3735920.27874280988005945No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA3735640.2787219186439606No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC3732610.27849584562474805No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA3722340.2777295849292652No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG3718790.2774647139001548No Hit
TTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAA3509330.26183657706706487No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG3466280.25862454951686664No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA3327720.24828637211023563No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3203770.23903826955861956No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA3187210.2378027021664876No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3163280.23601724759561085No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT3058570.22820467141021583No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA3024380.2256537022594312No Hit
GCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCTGTAGGCACCA2954580.2204458155462178No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG2943480.2196176272580134No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2904730.21672642940504752No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCTGTAGGCACCATCAA2853810.2129272088973566No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA2842480.21208185995092813No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCCTGTAGGCACCATCAATA2761270.2060226553666866No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA2757980.2057771833425251No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG2601870.19412957310184112No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA2476700.1847904444500801No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA2439550.18201862508910766No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA2421810.1806950160591305No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGACTGTAGGCACCATCAATA2206950.16466397681556275No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2190340.16342467884555595No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC2188620.16329634696666304No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2174680.16225626185517025No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCCTGTAGGCACCATCAATAG2163900.16145194926536455No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA2061660.15382366362698435No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2023780.15099737782903988No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2010030.14997146891347135No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1907740.14233945269721637No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGAT1842380.13746284129928477No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT1833790.13682192802039506No Hit
CTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1826710.1362936781933241No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCTGTAGGCACCATCAATAGA1763820.13160135734240735No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATCAA1759210.1312573980623513No Hit
TGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCGG1740070.1298293328518799No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1732320.1292510932812867No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1730940.12914812933194236No Hit
TGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAAT1726010.12878029435348762No Hit
TGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCTGTAGGCACC1702560.1270306533302089No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA1702260.12700826986296013No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA1667840.12444014005395147No Hit
GGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1624090.12117588441350612No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1622410.12105053699691301No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA1600870.11944340404845147No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1565660.11681633110902105No Hit
GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATA1562570.11658578139635872No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCCTGTAGGCACCATCAATA1560260.11641342869854322No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1497450.11172707677222612No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAA1460320.10895674964240358No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1437140.10722725373964878No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1397590.10427636664068617No Hit
GCACGAGCGCACGTGTTAGGACCCGAAACTGTAGGCACCATCAATAGATC1389200.10365037567329563No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA1370760.10227453855307135No Hit
GCTACACTGACTGGCTCAGCGTGTGCCCTGTAGGCACCATCAATAGATCG1346140.10043760200752244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)