FastQCFastQC Report
Tue 11 Jul 2023
SRR8136740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8136740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126496149
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT36325692.871683469193991No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG26360282.0838800396998645No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT23824301.8834012093127042No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG20392031.6120672574783286No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA17449731.379467291134689No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG14888091.1769599404958961No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG10920610.8633156097107747No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA10761610.8507460570993352No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG8699540.6877316083353652No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA7918670.6260008753309952No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT7297470.5768926609773709No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG7147240.5650164101043108No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT6237600.4931059205604749No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC5830590.46093023748888984No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC5590990.44198894940272054No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG5536120.4376512679449237No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT5447500.4306455210743214No Hit
ACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATCAATAGATC5439520.43001467183004916No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC5429580.42922887715735913No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA5225670.41310901883661294No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC5100860.40324231530558297No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGA5068650.40069599272939127No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG5042070.39859474299095066No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG4897340.3871532879629403No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA4856350.3839128731104692No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA4668370.3690523416645672No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCCTGTAGGCACCATCAATA4354940.34427451226202943No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA4303130.3401787354016603No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAAT4286310.3388490506537081No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG4197770.3318496280863064No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG4122220.32587711425112237No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT3935750.3111359540281341No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG3928770.3105841585738709No Hit
TTTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT3902050.30847184130482896No Hit
TGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCGG3837780.3033910542209471No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA3774260.298369557479572No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCTGTAGGCACCATCAATAGATCG3654440.28889733236068715No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGAT3622410.2863652394666971No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT3413610.26985880811280666No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA3385470.2676342344619519No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT3321990.26261589987217715No Hit
CTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG2979970.23557792261328048No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2919210.2307746143323304No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG2813620.22242732464527437No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG2769380.21892998497527383No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA2764210.21852127688092704No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC2707710.21405473774541547No Hit
CCTCTAGCATCACCAGTATTAGAGGCTGTAGGCACCATCAATAGATCGGA2679200.21180091419225736No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATCA2590230.2047674984951518No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA2477940.19589054841503517No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC2468280.1951268888035477No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA2453350.1939466157187125No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2408050.19036547903130235No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC2401450.18984372401724262No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG2390650.18898994308514483No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2369670.1873313945707549No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG2315620.1830585372207655No Hit
CCCCCCGAGTGTTACAGCCCCCCGGCTGTAGGCACCATCAATAGATCGGA2300980.18190118973503297No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAATAGATC2299890.18181502110392309No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2274060.17977306170798923No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2063330.16311405653938127No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCTGTAGGCACCATCAA2049750.16204050607105833No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG2044920.1616586762653146No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1947650.15396911411113393No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1869690.14780608064202808No Hit
TTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAA1801570.14242093646661133No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG1740470.13759074989705813No Hit
GCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGA1723260.1362302341710023No Hit
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1704240.1347266310850301Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAAT1697550.13419776122986954No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1695410.13402858611925014No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1685800.13326887919726313No Hit
TTTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1675370.13244434816746872No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC1650630.13048855740264473No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA1637760.1294711351252282No Hit
CACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATCG1637560.1294553243672264No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1636000.12933200045481227No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATC1626520.12858257052552644No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC1608590.1271651360706641No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1594000.126011741274432No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCCTGTAGGCACCATCAATAG1593630.12598249137212864No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1591120.12578406635920591No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1565010.12371997190206953No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1550430.12256736764373752No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1537510.12154599267682055No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGACTGTAGGCACCATCAATA1471500.1163276519983229No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA1465910.11588574131217227No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC1460450.11545410761872284No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGAT1421600.1123828678768711No Hit
TCTACACTGACTGGCTCAGCGTGTGCCCTGTAGGCACCATCAATAGATCG1416680.11199392323002655No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1407760.11128876342314578No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCTGTAGGCACCATCAATAGA1384770.10947131679083764No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCG1371850.10844994182392066No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGAT1370030.1083060639261042No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATC1339110.10586172073902424No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGATC1339110.10586172073902424No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1337970.10577159941841392Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1323460.10462452892538254No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA1282110.10135565470850816No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA1281900.10133905341260627No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA1274690.10076907558664097No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG1274150.1007263865400361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)