FastQCFastQC Report
Tue 11 Jul 2023
SRR8197267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8197267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30864215
Total Bases798.5 Mbp
Sequences flagged as poor quality0
Sequence length16-39
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCGCGGGGGCGCGCCGGC12017763.893752036136348No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC10215083.3096840467188295No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG8155822.6424841843539517No Hit
CCGGCCGAGGTGGGATCCCGAGGCC5597661.8136408134792996No Hit
TCACATTTTGAGGACATTTTGAG5586261.8099472155698761No Hit
CGGCCGAGGTGGGATCCCGAGGCC4035861.3076178998882688No Hit
CCCGCGGACGCTACGCCGCGACGAGT4012561.3000687041611134No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3687291.1946812838103933No Hit
CCCCCCGCGGGGGCGCGCCGGC3643811.1805937717839252No Hit
CCGCGGACGCTACGCCGCGACGAGT3528021.1430778330179465No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG3369431.0916947021007988No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG2787680.9032078087843802No Hit
TCGCTTCTGGCGCCAAGCGCCCGG2726040.8832364600881637No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2637790.8546434762717924No Hit
CACATTTTGAGGACATTTTGAG2413610.7820091973827943No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC2361930.7652648868600741No Hit
CCCCGCGGGGGCGCGCCGGC2131260.6905278491612374No Hit
CCCCCGCGGGGGCGCGCCGG2067540.6698825808464592No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC2011380.6516867511452988No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC1884370.6105355344368876No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC1872680.6067479765806453No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGG1703460.5519207276128681No Hit
CGGCCGGGCGCGACCCGCTCCGGGGAC1654740.5361354565473316No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1570420.5088157920102617No Hit
ACTGACCCGGTGAGGCGGGG1544450.5004015167727415No Hit
CCGCCGAGGGCGCACCACCGGCCCGT1511360.48968036284091465No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1483070.48051440802884504No Hit
ACATTTTGAGGACATTTTGAG1384880.44870086603530984No Hit
CCGGCCGAGGTGGGATCCCGAGGC1315970.4263740386722941No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC1281600.4152381649751986No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG1277660.41396160569773116No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC1243140.40277713202814325No Hit
ATTTTGAGGACATTTTGAG1232620.3993686539573419No Hit
CGCTTCTGGCGCCAAGCGCCCGG1225080.3969256953400564No Hit
AGTTTTATCCGGTAAAGCGAATGATT1110950.3599475962696605No Hit
TCCGGCCGAGGTGGGATCCCGAGGCC1000930.3243011364455568No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC999670.32389289667662047No Hit
CGCTTCTGGCGCCAAGCGCCCGGC959930.3110171439642965No Hit
TCGGCCGAGGTGGGATCCCGAGGCC954260.3091800650040832No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG933640.3024991887854591No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCC929770.3012453094951548No Hit
CGGCCGAGGTGGGATCCCGAGGC918790.29768779150871No Hit
ACTTCGAACGCACTTGCGGC912950.29579563257967195No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG852750.2762908436193825No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC815730.2642963704082543No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG805280.26091057232461606No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC796400.2580334539530651No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC771740.2500436184753119No Hit
TCGCTTCTGGCGCCAAGCGCCCG765410.24799269963613202No Hit
CGCCGAGGGCGCACCACCGGCCCGT760700.24646666049987018No Hit
CCCGCGGGGGCGCGCCGGC753830.2442407817597175No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT722700.2341546674684582No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC697600.226022272071394No Hit
TTTGAGGACATTTTGAG677610.2195455157372381No Hit
CCCCCCGCGGGGGCGCGCCGG642340.2081180422051881No Hit
CCACATTTTGAGGACATTTTGAG633830.20536080376578505No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT622790.20178384579034328No Hit
TACTTGGATAACTGTGGTAATTCT614800.1991950872555806No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG613400.19874148751231807No Hit
TCCCCGCGGGGGCGCGCCGGC598940.1940564501640492No Hit
ATCCGGTAAAGCGAATGATT597470.19358017043362355No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT593910.1924267310864702No Hit
GAGGGCGCACCACCGGCCCGTCTCGC592220.191879171396389No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG550940.17850445896647624No Hit
TTTTGAGGACATTTTGAG550830.17846881898664846No Hit
ACTTCGAACGCACTTGCGGCC536190.1737254616713887No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC535550.17351810178875438No Hit
CGGCCGAGGTGGGATCCCGAGGCCT530500.17188190271484305No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC512310.16598834605059612No Hit
AAATACATGCCGACGGGCGCTGACCCC502870.1629297877817401No Hit
ACTTCGAACGCACTTGCGGCCCCGGGT495760.1606261490855996No Hit
GCCGGCCGAGGTGGGATCCCGAGGC490540.15893487004286355No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT482480.15632343152093775No Hit
ACTCTGATCGTTTTTTC479930.1554972319885667No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG465680.1508802346017872No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC457250.14814891614771344No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC454220.14716719670336667No Hit
CCCCGCGGGGGCGCGCCGG453950.1470797167528803No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT449050.1454921176514614No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT443770.14378139861972838No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC440350.1426733192469013No Hit
CGAGGGCGCACCACCGGCCCGTCTCG439040.14224887948713422No Hit
CTCGCTTCTGGCGCCAAGCGCCCG434180.14067424037837994No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC425590.1378910819536476No Hit
CGCCGGCCGAGGTGGGATCCCGAGGC425460.13784896197748753No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG417190.13516948349407235No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT411310.13326436457236968No Hit
ACTTTGAAAAAATTAGAGTGTTC403970.13088620591840747No Hit
ATGTTAGGGATAACAGGG400510.12976516655291573No Hit
AACCTCGGGCCGATCGC399460.12942496674546883No Hit
AAATACATGCCGACGGGCGCTGACCC390120.12639880845827442No Hit
CCGCCGAGGGCGCACC389430.1261752485848093No Hit
CGCGGACGCTACGCCGCGACGAGT388090.12574108883054372No Hit
CCCCGCGGACGCTACGCCGCGACGAGT387300.125485128975417No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC385570.12492460929267113No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC384760.1246621694412121No Hit
TATCCGGTAAAGCGAATGATT383690.12431548963743287No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCT373760.12109817145843495No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT367610.11910557258624592No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT360500.1168019338901054No Hit
AACGATGCCGACCGGCGATGCGGCGGCG359800.11657513401847415No Hit
ACTTCGAACGCACTTGCGGCCCCGGG356960.1156549745392844No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT356230.11541845467315465No Hit
CCGCCGAGGGCGCACCACCGGCCC354040.1147088950747654No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCTCC351010.11372717563041859No Hit
CCCCCCCGCGGGGGCGCGCCGGC345270.11186741668304215No Hit
GAGGGCGCACCACCGGCCCGTCTCG326710.10585398008664729No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC324740.10521570044791356No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT324430.10511526050476255No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGC324410.10510878050843024No Hit
AGCGTTTACTTTGAAAAAATT322870.10460982079084143No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG321170.10405902110259405No Hit
CGCTTCTGGCGCCAAGCGCCCG320170.10373502128597795No Hit
TCAACCTATTCTCAAACTTTAAATGGGT318070.10305462167108413No Hit
ACTTGGATAACTGTGGTAATTCT317520.10287642177194528No Hit
AACCCGGCGGCGGGTCCGGCCGTG314780.10198866227441716No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT314420.10187202234043535No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT310300.10053714309597701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)