FastQCFastQC Report
Tue 11 Jul 2023
SRR8197269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8197269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50248884
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length16-39
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCGCGGGGGCGCGCCGGC33749636.716493444909144No Hit
CCCCCCGCGGGGGCGCGCCGGC23471874.6711226462263316No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC16687283.3209254955791656No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG8401661.672009272882558No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG6816921.3566311243847724No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC6546521.3028189839997242No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC6429941.2796184687405197No Hit
CCGGCCGAGGTGGGATCCCGAGGCC5881391.1704518651598312No Hit
CCCCGCGGGGGCGCGCCGGC5249061.0446122544731542No Hit
TCGCTTCTGGCGCCAAGCGCCCGG4941420.9833890042214669No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC4742380.9437781742575616No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG4641680.9237379281896092No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC4541180.9037374840006397No Hit
CCCCCGCGGGGGCGCGCCGG4245490.8448923960181882No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG4188480.8335468704140772No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC3592510.7149432413265138No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG3579200.7122944262801937No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC3184480.6337414379192978No Hit
CCCCCCGCGGGGGCGCGCCGG3026550.6023118841803532No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2945480.5861781925345844No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC2785840.5544083327303349No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2752710.547815151476797No Hit
CGGCCGAGGTGGGATCCCGAGGCC2562150.509891921181772No Hit
TCACATTTTGAGGACATTTTGAG2455160.48859990601980335No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG2196780.43717985856163494No Hit
ACTTTGAAAAAATTAGAGTGTTC2090140.4159574966878866No Hit
CCCCCCCGCGGGGGCGCGCCGGC2081450.41422810504607427No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC2021550.4023074422906586No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCC1984550.3949440946787992No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC1913180.38074079416370715No Hit
TCGCTTCTGGCGCCAAGCGCCCG1714500.3412016075819714No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG1688390.3360054722807376No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1656290.3296172707039623No Hit
CTCGCTTCTGGCGCCAAGCGCCCG1584180.3152667032366331No Hit
AGTTTTATCCGGTAAAGCGAATGATT1503290.2991688332819491No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1469630.2924701770491062No Hit
TCCCCGCGGGGGCGCGCCGGC1465650.291678119657344No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC1358230.27030053045556196No Hit
ATTGATCATCGACACTTCGAACGCA1348620.26838805017042766No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT1335260.2657292846543617No Hit
CCCGCGGGGGCGCGCCGGC1285440.25581463659969045No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1265740.2518941515198626No Hit
ATCCGGTAAAGCGAATGATT1253640.24948613784138968No Hit
CGCTTCTGGCGCCAAGCGCCCGG1233640.24550594994308728No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT1223770.2435417272152751No Hit
CACATTTTGAGGACATTTTGAG1179190.23466988838995906No Hit
AGCGTTTACTTTGAAAAAATT1173770.23359125746951911No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1128490.22458011206776254No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1102870.2194814913700372No Hit
TCCGGCCGAGGTGGGATCCCGAGGCC1069170.2127748747613977No Hit
ACTTCGAACGCACTTGCGGCCCCGGGT1017280.20244827725925219No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1009210.20084227144228717No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC998610.1987327718561869No Hit
CCGGCCGAGGTGGGATCCCGAGGC997540.1985198318036277No Hit
CGCCGGCCGAGGTGGGATCCCGAGGC948920.18884399502285465No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG929170.18491355947328103No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGG914040.18190254732821529No Hit
CGCGCCGTCGGGCCCGGGGG883730.17587057256833802No Hit
ACTTCGAACGCACTTGCGGC883020.1757292758979483No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC854830.1701192010552911No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC841480.16746242563317426No Hit
CGCTTCTGGCGCCAAGCGCCCGGC830150.16520764918878597No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT823340.163852395209414No Hit
ACTGACCCGGTGAGGCGGGG819500.16308819913293995No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG819280.1630444170660586No Hit
TCCCCCGCGGGGGCGCGCCGGC815730.16233793371410996No Hit
CCCCCGCGTGGGCGCGCCGGC814600.16211305309785587No Hit
CCCCGCCCCTTGCCTCTCGGCG813300.1618543408844662No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGC810880.16137273814877162No Hit
ACTTCGAACGCACTTGCGGCCCCGGG810690.16133492636373775No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCG807880.16077570996402626No Hit
AACCTCGGGCCGATCGC802290.15966324744645075No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT797240.15865825000212938No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC795450.15830202318523134No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC782940.1558124156548432No Hit
TGGGGGGGCCGGGCCACCCCTCCC763030.15185013860208318No Hit
GCCGGCCGAGGTGGGATCCCGAGGC746230.14850678076750917No Hit
CCGCCGAGGGCGCACCACCGGCCCGT742270.14771870356364533No Hit
TCGCTGCGATCTATTGAAAGTC741600.1475853672690522No Hit
TACTTGGATAACTGTGGTAATTCT700360.13937821982275267No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT687140.13674731562197479No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG677810.13489055796741675No Hit
CCCCGCGGGGGCGCGCCGG664970.1323352773367066No Hit
ACATTTTGAGGACATTTTGAG661080.1315611307904868No Hit
TCGGCCGAGGTGGGATCCCGAGGCC659290.13120490397358875No Hit
ATTTTGAGGACATTTTGAG657020.13075315264713142No Hit
AACCTCGGGCCGATCGCACGCCC641910.12774612068996397No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG640500.12746551744313364No Hit
CTTTGGTGACTCTAGATAACCTC636320.12663365817238847No Hit
ATGCGTGCATTTATCAGA624690.12431917890952564No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC624610.12430325815793242No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC624270.12423559496366127No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG619990.12338383475342457No Hit
AAAGCGAATGATTAGAGGTC616970.12278282638078092No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC605800.12055989143957904No Hit
GAGGGCGCACCACCGGCCCGTCTCGC605600.12052008956059601No Hit
CCGCCGAGGGCGCACC591420.11769813634069963No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT591150.11764440380407255No Hit
TATCCGGTAAAGCGAATGATT584090.11623939747597181No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT581580.11573988389473484No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC573300.11409208610483766No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT573040.11404034366215975No Hit
ACTTCGAACGCACTTGCGGCC572320.11389705689782086No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCCT552820.11001637369697603No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC551540.1097616416714847No Hit
TTTTGGTGACTCTAGATAACCTC549660.10938750400904428No Hit
ATTGATCATCGACACTTCGAACGC504710.10044203170760967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)