FastQCFastQC Report
Tue 11 Jul 2023
SRR8197270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8197270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83682833
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length16-39
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCGCGGGGGCGCGCCGGC36633374.377644576158171No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC36484824.359893026088158No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG23178312.7697807506110603No Hit
CCCCCCGCGGGGGCGCGCCGGC14757231.7634716071335683No Hit
CCGGCCGAGGTGGGATCCCGAGGCC13055301.5600929762977789No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC10510491.2559911780233348No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC9169291.0957193573979505No Hit
TCGCTTCTGGCGCCAAGCGCCCGG8151070.9740432664367373No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG7250400.8664142620506167No Hit
CGGCCGAGGTGGGATCCCGAGGCC7108570.849465744067245No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC7089110.8471402969830144No Hit
CCCCCGCGGGGGCGCGCCGG6288060.7514157652860534No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC5722720.6838583010209514No Hit
CCCCGCGGGGGCGCGCCGGC5708860.6822020473422548No Hit
AGTTTTATCCGGTAAAGCGAATGATT5585790.6674953272674218No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC5556490.6639940117706101No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC5420200.6477075172634272No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG5234600.6255285358228729No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG4784890.5717887203938232No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC4512280.539212146414785No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG4461000.5330842468012525No Hit
TCACATTTTGAGGACATTTTGAG4163640.497550076967399No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC4053170.48434904205501744No Hit
AGCGTTTACTTTGAAAAAATT3926600.4692240761017257No Hit
ATCCGGTAAAGCGAATGATT3856630.46086274349722356No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC3644780.43554691796822886No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC3536330.4225872706771292No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCC3266160.39030227382478794No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC3232700.3863038432267225No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC3099320.3703650902927725No Hit
CCGCCGAGGGCGCACCACCGGCCCGT2948680.35236378768390886No Hit
CCCCCCGCGGGGGCGCGCCGG2857330.34144757025613603No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC2774410.3315387279013367No Hit
CCGGCCGAGGTGGGATCCCGAGGC2671570.3192494690039951No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2640670.3155569553913166No Hit
ACTGACCCGGTGAGGCGGGG2613810.31234721702120194No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG2605320.3113326720188835No Hit
TACTTGGATAACTGTGGTAATTCT2493750.29800018840184345No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC2313490.2764593306729948No Hit
TCCGGCCGAGGTGGGATCCCGAGGCC2272980.27161843337689107No Hit
TCGCTTCTGGCGCCAAGCGCCCG2269460.271197797521984No Hit
TCGGCCGAGGTGGGATCCCGAGGCC2179170.26040824884597297No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT2175790.26000434282620427No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT2082100.2488084981539762No Hit
ATTGATCATCGACACTTCGAACGCA2065700.24684871746634102No Hit
CCCGCGGACGCTACGCCGCGACGAGT2053710.24541592658556383No Hit
CACATTTTGAGGACATTTTGAG2050430.2450239704480368No Hit
AAATACATGCCGACGGGCGCTGACCCC1934240.23113940227143123No Hit
CGCTTCTGGCGCCAAGCGCCCGG1932920.23098166382584107No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1927690.23035668498460135No Hit
ACTTTGAAAAAATTAGAGTGTTC1868170.2232441150743546No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1843510.22029727411355685No Hit
TATCCGGTAAAGCGAATGATT1790540.21396742149014003No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1762690.21063937928583273No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1743380.20833185702496473No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1734760.20730177717573206No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC1729170.2066337787584223No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1710370.2043872008969868No Hit
TCCCCGCGGGGGCGCGCCGGC1700270.20318026279057738No Hit
AAATACATGCCGACGGGCGCTGACCC1698320.20294724008686466No Hit
GCCGGCCGAGGTGGGATCCCGAGGC1685000.20135551577227315No Hit
CTCGCTTCTGGCGCCAAGCGCCCG1683570.20118463245621718No Hit
AAATTCTAGAGCTAATACATGCC1652020.19741444460896776No Hit
CCCGCGGGGGCGCGCCGGC1615330.19303003281449613No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1564130.18691169310675704No Hit
CGCCGAGGGCGCACCACCGGCCCGT1535750.18352031652656883No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1504730.17981346305520035No Hit
AAAGCGAATGATTAGAGGTC1497200.17891363692240198No Hit
CCGCGGACGCTACGCCGCGACGAGT1480250.1768881318824376No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT1468220.17545056104876375No Hit
CGCCGGCCGAGGTGGGATCCCGAGGC1442990.1724356057591884No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC1418880.16955448915071983No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1418380.16949473973951146No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG1409390.1684204453259846No Hit
CCCCCCCGCGGGGGCGCGCCGGC1396110.16683350096428978No Hit
CGGCCGAGGTGGGATCCCGAGGC1386780.16571857695114123No Hit
ACTTCGAACGCACTTGCGGC1384210.1654114649775301No Hit
CGCTTCTGGCGCCAAGCGCCCGGC1373430.1641232676718772No Hit
ACTTCGAACGCACTTGCGGCCCCGGGT1370970.16382930056873193No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1364610.1630692880581612No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG1346250.16087528967858916No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG1337730.1598571597115982No Hit
TAGCGTTTACTTTGAAAAAATT1305890.15605231720584795No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1299580.15529827963639806No Hit
TCGCTGCGATCTATTGAAAGTC1277610.15267289050790142No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1271970.15199891714947078No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGG1228360.14678757350387503No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1200020.1434009768765835No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1159450.1385529096511348No Hit
ACTTCGAACGCACTTGCGGCCCCGGG1143910.13669589795077805No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1134880.1356168235843545No Hit
CGGCCGAGGTGGGATCCCGAGGCCT1132680.13535392617503758No Hit
ACATTTTGAGGACATTTTGAG1097450.13114398266129448No Hit
CCCCGCGGGGGCGCGCCGG1080610.1291316224917959No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1059740.1266376820679577No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT1052470.12576892562898773No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG1049720.1254403038673416No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG1015720.12137734390517108No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1012040.12093758823867734No Hit
ATTTTGAGGACATTTTGAG1010200.12071771040543047No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG1009890.12068066577048127No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGC983710.11755218659960998No Hit
ACTTCGAACGCACTTGCGGCC970500.11597360715548431No Hit
GCGTTTACTTTGAAAAAATT951420.11369356962377217No Hit
GAGGGCGCACCACCGGCCCGTCTCG947920.11327532374531345No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC939820.11230738328373753No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCTCC912470.10903909049063863No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCCT890020.10635634192738193No Hit
ACTTGGATAACTGTGGTAATTCT883440.10557003967587952No Hit
ACCCTACGCCGGCAGGCGCGGG866900.10359352915310599No Hit
ACTCTGATCGTTTTTTC850960.10168871792378253No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC850470.10163016350079832No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC844820.10095499515414351No Hit
CGAGGGCGCACCACCGGCCCGTCTCG840320.10041725045326799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)