FastQCFastQC Report
Mon 10 Jul 2023
SRR8197271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8197271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37588636
Total Bases944.2 Mbp
Sequences flagged as poor quality0
Sequence length16-39
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCGGACGCTACGCCGCGACGAGT7886232.0980356935537645No Hit
CCCCCGCGGGGGCGCGCCGGC6506041.7308529099060683No Hit
CCGCGGACGCTACGCCGCGACGAGT5817891.5477789617053408No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC5036691.3399501913291028No Hit
ATTGATCATCGACACTTCGAACGC4717211.2549564182110784No Hit
ATTGATCATCGACACTTCGAAC4289831.141257160807857No Hit
TCACATTTTGAGGACATTTTGAG3959451.0533635750975374No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG3870321.029651621303843No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGG3737900.9944228888752441No Hit
CCGGCCGAGGTGGGATCCCGAGGCC2981140.7930960836142074No Hit
CCGCCGAGGGCGCACCACCGGCCCGT2583980.6874364901136609No Hit
AGTTTTATCCGGTAAAGCGAATGATT2489080.6621894979110176No Hit
CCCCCCGCGGGGGCGCGCCGGC2407670.6405313563386551No Hit
ACCCACTCCCGACCCGGGGAGGT2335650.6213713102013065No Hit
ACTGACCCGGTGAGGCGGGG2041300.5430630683167115No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC1973030.5249006641262535No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1972170.5246718715730999No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1942090.5166694529697752No Hit
CGGCCGAGGTGGGATCCCGAGGCC1933270.5143229991106887No Hit
ACGGGGTCGGCGGCGACGTCGGCT1916340.5098189782677935No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC1912190.5087149211799014No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1841750.48997521484950934No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGT1803500.47979926699122577No Hit
TACTTGGATAACTGTGGTAATTCT1734430.46142403251876446No Hit
CACATTTTGAGGACATTTTGAG1675990.44587678041842227No Hit
AGATCCCCGAATCCGGAGT1599760.42559671492203127No Hit
AGCGTTTACTTTGAAAAAATT1443720.3840841684172844No Hit
CGGCCGGGCGCGACCCGCTCCGGGGAC1419170.3775529391383077No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC1406450.3741689376544549No Hit
ACTTGGATAACTGTGGTAATTCT1402250.3730515786739375No Hit
CCCCGCGGGGGCGCGCCGGC1278480.3401240736694995No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1259020.3349469770597688No Hit
ATGTTAGGGATAACAGGG1233950.3282774080974899No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC1191610.3170133654224644No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1183920.3149675343367075No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGC1132890.301391622723421No Hit
ATCCGGTAAAGCGAATGATT1119100.2977229607373888No Hit
TCGCTTCTGGCGCCAAGCGCCCGG1112500.2959671109108615No Hit
ACATTTTGAGGACATTTTGAG1076050.2862700311870854No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCT1028940.2737369879556151No Hit
AAATACATGCCGACGGGCGCTGACCCC1015780.27023592981666056No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT1004170.26714723034908744No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC978910.2604271141948327No Hit
AGAATCCCGCCCAGGCGGAACGAT962050.25594171600161286No Hit
CCGGCCGAGGTGGGATCCCGAGGC943430.25098809118798565No Hit
GGGCCGCCGGTGAAATACCACT927660.2467926742539953No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCT924140.2458562210131807No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC922330.24537469250014818No Hit
CCCCCGCGGGGGCGCGCCGG895060.23811984026236013No Hit
ACGTGCGCGAGTCGGGGGCTCGC880970.23437136692057675No Hit
ATTTTGAGGACATTTTGAG842280.22407836240719137No Hit
ACCCGGCCGTCGCCGGCAGTCGAGA837160.22271624860237013No Hit
ATTCGAACGTCTGCCCT827660.22018888900358075No Hit
ACTCCCGACCCGGGGAGGT824440.21933224711851743No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT800970.21308833872024513No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG779240.20730733618533007No Hit
CCCCGCGGACGCTACGCCGCGACGAGT769950.20483584453556658No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT762410.20282991912768528No Hit
CGCCGAGGGCGCACCACCGGCCCGT760450.20230848493677714No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT749290.19933950250283092No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC742560.197549067755478No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCC740030.19687599198864253No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG720010.19154991418150955No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC719350.1913743291988568No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT693320.1844493638981739No Hit
AGGAGGGCCGCTGCGGTGAGCCTT682940.1816878909891809No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC681470.18129681534599978No Hit
AAATACATGCCGACGGGCGCTGACCC669390.17808307808774973No Hit
ACTCTGATCGTTTTTTC651620.17335558544875107No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT639140.1700354330494993No Hit
ACTTTGAAAAAATTAGAGTGTTC633370.16850039464055042No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCG621270.16528133662525024No Hit
TATCCGGTAAAGCGAATGATT603620.16058576852855208No Hit
TCGGCCGAGGTGGGATCCCGAGGCC602440.16027184386259719No Hit
TCCGGCCGAGGTGGGATCCCGAGGCC592120.1575263332247544No Hit
CGGCCGAGGTGGGATCCCGAGGC591570.15738001240587715No Hit
ACTTCGAACGCACTTGCGGCCCCGGGT591330.15731616332127615No Hit
TACTTGGATAACTGTGGT587770.1563690685663614No Hit
AAAGCGAATGATTAGAGGTC578240.1538337278319969No Hit
GCGTTTACTTTGAAAAAATT577820.15372199193394515No Hit
CGGCCGAGGTGGGATCCCGAGGCCT570030.15164955706293784No Hit
ACCCGGCCGTCGCCGGCAGTCGAG563160.14982187701623437No Hit
TCGCTGCGATCTATTGAAAGT560350.1490743106506977No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC557490.14831344239253585No Hit
TAGCGTTTACTTTGAAAAAATT553760.14732112120269542No Hit
AACCTCGGGCCGATCGC538610.1432906477372576No Hit
GCCGGCCGAGGTGGGATCCCGAGGC528370.14056642012761517No Hit
ACTTCGAACGCACTTGCGGC514530.13688445624895781No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC513410.13658649385415314No Hit
AACCCGGCGGCGGGTCCGGCCGTGT505060.13436507778574355No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC502790.13376117186055914No Hit
AACCCGGCGGCGGGTCCGGCCGTG501940.1335350396859306No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTC500450.13313864328569944No Hit
AACCCGGCGGCGGGTCCGGCCGTGTC493410.13126573680407025No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT489400.13019892501552863No Hit
AAGTGGCGTTCAGCCACCCGAGATT486290.12937154729424075No Hit
TACTTGGATAACTGTGGTAATT484670.12894056597318404No Hit
TAACCCGGCGGCGGGTCCGGCCGTGTCGG480200.1277513767724905No Hit
TTGATCATCGACACTTCGAACGC476560.1267829989893754No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGC470840.1252612624730517No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT465630.12387520526150511No Hit
CGCGGACGCTACGCCGCGACGAGT462740.12310635586776812No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG451960.12023846781777343No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTC450230.11977822233294126No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC449440.11956805242946299No Hit
ACTTCGAACGCACTTGCGGCCCCGGG442110.1176179949706076No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT440660.1172322400844766No Hit
ACAGTGAAACTGCGAATGGCTCATT438250.11659108885994161No Hit
ATCGACACTTCGAACGC434550.11560674880567627No Hit
ACGTGCGCGAGTCGGGGGCTCGCAC433060.1152103524054451No Hit
AACCTCGGGCCGATCGCACGCCC432940.1151784278631446No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC432800.11514118256379402No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCTCC428690.11404776699000198No Hit
ACTTGCGGCCCCGGGT427550.11374448383814725No Hit
CCTCCCGGGGCTACGCCTGT426220.11339065349431673No Hit
AACCTCGGGCCGATCGCAC418840.11142729414283614No Hit
AACGATGCCGACCGGCGATGCGGCGGCG412790.10981776513518608No Hit
ATTGATCATCGACACTTC412500.10974061415795987No Hit
CTCCCGGGGCTACGCCTGT410110.10910478369047497No Hit
GAGGGCGCACCACCGGCCCGTCTCGC403990.1074766320331496No Hit
CCACATTTTGAGGACATTTTGAG401200.10673438642466303No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG400670.10659338636283583No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC398910.10612515974242855No Hit
AGATAACCTCGGGCCGATCGCAC397210.1056728953931715No Hit
ACAGTGAAACTGCGAATGGCTC396090.10537493299836685No Hit
TCGCTTCTGGCGCCAAGCGCCCG395110.10511421590291278No Hit
TTGATCATCGACACTTCGAAC392350.10437995143000134No Hit
TTTGAGGACATTTTGAG391550.10416712114799803No Hit
AGATCCCCGAATCCGGAGTG391070.10403942297879604No Hit
GAGCGCCGTTCCGAAGGGACGGGC389910.10373081906989122No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGC387270.10302847913928029No Hit
AAATCGGTCGTCCGACCTGGGT387080.10297793194730451No Hit
CCAACTTCTTAGAGGGAC386720.10288215832040301No Hit
AGTGAAACTGCGAATGGCTCATT385570.10257621479002324No Hit
CTACTTGGATAACTGTGGT384490.10228889390931875No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA384310.10224100709586802No Hit
CGCTTCTGGCGCCAAGCGCCCGG382460.10174883706873535No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT380780.10130189347652839No Hit
CCCGCGGACGCTACGCCGCGAC379980.10108906319452507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)