FastQCFastQC Report
Tue 11 Jul 2023
SRR8197273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8197273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74507030
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length12-37
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCGCGGGGGCGCGCCGGC40053375.3757840032007715No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC38060945.108368968673157No Hit
CCCCCCGCGGGGGCGCGCCGGC30345524.072839838066287No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG27889383.7431877233597954No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC20681512.7757796814609303No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG10063231.350641677704775No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT7307550.9807866452333425No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCG4685000.6287997253413536No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG4665370.6261650746245019No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4505670.6047308555984583No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCC4477750.6009835581957836No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4436310.5954216669218999No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC4361940.5854400584750191No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC4031970.5411529623446271No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCG4016480.5390739638930716No Hit
CCGGCCGAGGTGGGATCCCGAGGCC3724280.49985618806708576No Hit
TCGCTTCTGGCGCCAAGCGCCCGG3673450.49303401303205885No Hit
GCGCAGCGGAAGCGTGCTGGGC3495160.4691047274331026No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC3441520.4619054067783939No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC3356690.4505199039607404No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCCT3278400.4400121706636273No Hit
CCCCCGCGGGGGCGCGCCGG3257430.4371976711459308No Hit
TACCCCCGCGGG3093220.41515814011107405No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC3053810.4098687063489177No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGC3050070.40936674029282877No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC2971620.3988375325120328No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG2969810.39859460241536937No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC2881130.38669236983409483No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC2868300.3849703846737684No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC2849770.38248337103223684No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2790300.37450157387833066No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG2743460.36821491878014734No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG2718330.3648420826866941No Hit
CGGCCGAGGTGGGATCCCGAGGCC2690980.36117128813214No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC2677440.35935400995047045No Hit
TCCCCCCCGCGGGGGCGCGCCGGC2628560.3527935551853295No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG2346420.3149259875209091No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC2336020.3135301460815174No Hit
CCCCGCGGGGGCGCGCCGGC2194270.2945050956936547No Hit
ACCCCCGCGGGG2157250.28953643703151233No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT2144640.28784397928624994No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2125890.28532743822965434No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT2115890.28398528299946996No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG2099890.28183783463117507No Hit
ATGCGTGCATTTATCAGA2087000.2801077965394675No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2024390.27170456264328346No Hit
GGGCGCGCCGGC2018840.27095966649053116No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT1988300.26686072441754827No Hit
TTGCGTGCATTTATCAGA1980510.2658151854932347No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1956220.26255509043911696No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC1930380.2590869613243207No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG1929230.25893261347284946No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT1863680.25013478593899124No Hit
CCCGCGGGGGCGCGCCGGC1793050.2406551435481994No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG1757990.23594954731117318No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC1671190.2242996399131733No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCCTC1660650.22288500830055905No Hit
TGCGTGCATTTATCAGA1598430.21453411845835219No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCC1551800.20827564862000272No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1486130.1994617152233823No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCG1444140.1938260054118383No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCCG1438510.19307037201724456No Hit
CCCCCCCGCGGGGGCGCGCCGGC1404110.1884533580254105No Hit
CGCTTCTGGCGCCAAGCGCCCGG1388360.18633946353787018No Hit
CCCCCGCCCCTTGCCTCTCGGC1300440.17453923475408967No Hit
CGCTTCTGGCGCCAAGCGCCCGGC1276310.1713006141836549No Hit
CCCCCCCCCGCGGGGGCGCGCCGGC1275420.17118116236816847No Hit
GCGGGGCGGACTGT1222960.16414021603062154No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1219950.16373622730633605No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1212380.16272021579708654No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1205140.1617484954104331No Hit
CCCCCCGCGGGGGCGCGCCGG1194460.1603150736245962No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTC1172000.15730059297760224No Hit
CCCCCGCCCCTTGCCTCTCGGCG1162310.15600004455955363No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGG1154640.1549706114980023No Hit
ACCCCCCCCGCGGGGGCGCGCCGGC1140830.15311709512511773No Hit
CCCCCCCCCCGCGGGGGCGCGCCGGC1130010.15166488316605828No Hit
TCGGCCGAGGTGGGATCCCGAGGCC1113060.14938993005089587No Hit
CCCCGCCCCCGG1112470.149310742892315No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1111510.1491818959902173No Hit
TGTGCATTTATCAGATCAAAACCA1106130.14845981647637813No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGC1097330.14727871987381594No Hit
CGGCCGAGGTGGGATCCCGAGGCCT1081540.1451594567653549No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1076440.1444749575979609No Hit
CTCCCCCCCGCGGGGGCGCGCCGGC1070130.14362805764771458No Hit
AGTGGGGGGGCCGGGCCACCCCTCCCACGGCG1055520.14166716885641528No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACG1041120.13973446532494987No Hit
CCGCGGGGGCGCGCCGGC1033040.13865000389896096No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1026570.1377816294650317No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1026120.1377212324796734No Hit
TGCGGGGCGGACTGT1019320.13680856692314805No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC1016970.13649316044405474No Hit
TCGATGCCGACCGGCGATGCGGCGGCG1008950.1354167519494469No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC1005200.1349134437381278No Hit
CCGCCGAGGGCGCACC976580.1310721954693403No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG877320.11774996265453072No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGC870810.11687621959968074No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC870780.11687219313399018No Hit
TCGCTTCTGGCGCCAAGCGCCCG865240.11612863913646805No Hit
ACCCCCCCGCGGGGGCGCGCCGGC854240.11465226838326531No Hit
TCCCCGCGGGGGCGCGCCGGC841610.11295712632754251No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGG825960.11085665339230406No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT809800.1086877305403262No Hit
AGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC798970.10723417642603658No Hit
CCCCCGCGGGGGCGC787320.10567056558287184No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT779260.10458878846734329No Hit
GACCCCCGCGGG770870.10346272022921865No Hit
TCCCCCGCGGGGGCGCGCCGGC757610.10168302239399424No Hit
CCCCCCCCGCGGGGGCGCGCCGGC757100.10161457247725483No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC756980.10159846661449262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)