FastQCFastQC Report
Fri 7 Jul 2023
SRR8239838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8239838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12264129
Total Bases919.8 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAA6264285.107806677506409RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC5998214.890856904717816RNA PCR Primer, Index 1 (100% over 24bp)
ACACACGGCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC3442872.807268253619968RNA PCR Primer, Index 1 (100% over 36bp)
TCGACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG3213332.620104534125497RNA PCR Primer, Index 1 (100% over 29bp)
AAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGA1657441.3514534949852532RNA PCR Primer, Index 1 (100% over 22bp)
AACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAAC1109130.9043691565866602RNA PCR Primer, Index 1 (100% over 24bp)
AACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGA1087290.8865611247239816RNA PCR Primer, Index 1 (100% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGAA1076000.8773554159451519RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACT927730.756458122708918RNA PCR Primer, Index 1 (100% over 25bp)
ACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA834980.6808310643177351RNA PCR Primer, Index 1 (100% over 32bp)
TGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAAC759220.6190574153288831No Hit
ACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAA639290.5212681634382678No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTGCC588590.4799280894713355No Hit
CGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACT510620.41635243725828386RNA PCR Primer, Index 1 (100% over 25bp)
AGAGAACTCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC505920.41252012270908106RNA PCR Primer, Index 1 (100% over 36bp)
ATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCC385680.31447810113543323No Hit
AAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTGC378130.3083219362744798No Hit
TTGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG359240.29291929333098177RNA PCR Primer, Index 1 (100% over 39bp)
TAAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGG357780.291728829662506Illumina Small RNA Adapter 2 (100% over 21bp)
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTT356240.29047313510808637No Hit
ATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACTCC348600.28424358550044604RNA PCR Primer, Index 1 (100% over 27bp)
GATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACTC343270.27989757772443524RNA PCR Primer, Index 1 (100% over 26bp)
TGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA329080.26832724933013996RNA PCR Primer, Index 1 (100% over 32bp)
AAACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGG314040.256063842772691Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAAC308210.25131014195953094RNA PCR Primer, Index 1 (100% over 24bp)
TTCGACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCA288580.235304113321052RNA PCR Primer, Index 1 (100% over 28bp)
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCT288280.23505949749876245No Hit
AGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA281870.22983287276250927RNA PCR Primer, Index 1 (100% over 37bp)
GTAAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAG271940.22173608904472547No Hit
ATGTTGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC257540.20999452957482756RNA PCR Primer, Index 1 (100% over 36bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCA254490.2075076020482172No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTTGGAATTCTCGGG239750.1954888113130578No Hit
AGAACTCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC234800.19145265024528038RNA PCR Primer, Index 1 (100% over 38bp)
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCTGGAATT221810.18086078514014325No Hit
AAATTGAAGAGTTTGATCATGGCTTGGAATTCTCGGGTGCCAAGGAACTC204550.16678722149775171RNA PCR Primer, Index 1 (100% over 26bp)
AAACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAA198610.1619438282164188RNA PCR Primer, Index 1 (100% over 23bp)
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTA189810.15476843076259228No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGGAATTCTCGGGTGCC185010.1508545776059596No Hit
ACGTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAA181240.1477805721058544No Hit
TGTTGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA180260.1469814937530419RNA PCR Primer, Index 1 (100% over 37bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGTGGAATTCTCGGGTGCCAAGGA176210.1436791801521331RNA PCR Primer, Index 1 (100% over 22bp)
ATGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC172760.1408660981958034RNA PCR Primer, Index 1 (100% over 31bp)
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTG172680.14080086730985952No Hit
AGGGCGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA170720.1392027106042345RNA PCR Primer, Index 1 (100% over 40bp)
AGGCGCTTGAGAGAACTCGGGTGAAGGTGGAATTCTCGGGTGCCAAGGAA166530.13578624295292394RNA PCR Primer, Index 1 (100% over 23bp)
TGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGTGG164280.13395162428575239No Hit
TGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGTGGAATTCTCGGGTG161550.13172562030291757No Hit
TGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCTGGAATTCTCGG160020.1304780796092409No Hit
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCATGGAATTCTCGGG156660.12773838239959803No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCTGGAATTCTCGGGTG156460.12757530518473836No Hit
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCTGGAATTCTCGGGT153750.12536560892338952No Hit
CGACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT151790.12376745221776451RNA PCR Primer, Index 1 (100% over 30bp)
ACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTG151000.12312329721906871No Hit
ACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTG150310.12256068082780278No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTTGGAATTCTCGGGTGCCAA149980.12229160342328428No Hit
AAGGCTGAGGCGTGATGACGAGGCACTTGGAATTCTCGGGTGCCAAGGAA149020.12150883279195776RNA PCR Primer, Index 1 (100% over 23bp)
CGTAAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAA141380.11527928318431746No Hit
ACTAACTGGAGGACCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA140770.1147818976789954RNA PCR Primer, Index 1 (100% over 34bp)
TGCAAGTCGAACGGTAACAGGAAGAAGCTGGAATTCTCGGGTGCCAAGGA138590.11300435603702472RNA PCR Primer, Index 1 (100% over 22bp)
TCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGGCAAGACG135350.11036250515629768No Hit
CGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTC132010.1076391156681408RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCTGGAATTCTCGGGTG124620.10161341257907512No Hit
ATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGTGGAATTCTCGG123140.1004066411891134No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)