FastQCFastQC Report
Mon 10 Jul 2023
SRR8239839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8239839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11366612
Total Bases852.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC202968217.856525761590174RNA PCR Primer, Index 1 (100% over 24bp)
AACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAA196519617.28919751989423RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGAA5503114.841469032285082RNA PCR Primer, Index 1 (100% over 23bp)
AACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGA5326834.68638324242967RNA PCR Primer, Index 1 (100% over 22bp)
AAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGA3469263.052149576320543RNA PCR Primer, Index 1 (100% over 22bp)
AACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAAC1926141.6945594694355712RNA PCR Primer, Index 1 (100% over 24bp)
ACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACT1703281.4984940103524251RNA PCR Primer, Index 1 (100% over 25bp)
CGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACT1429611.2577274565191456RNA PCR Primer, Index 1 (100% over 25bp)
TGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1420251.249492812810009RNA PCR Primer, Index 1 (100% over 32bp)
AAACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGG1320421.1616654109421523Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTGCC881810.7757896548241464No Hit
ACACACGGCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA813350.7155606261566771RNA PCR Primer, Index 2 (100% over 36bp)
TAAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGG808660.7114345066058382Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGAACTCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA654330.5756596600640542RNA PCR Primer, Index 2 (100% over 36bp)
AAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTGC629710.5539997318462176No Hit
ACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA627860.5523721580361853RNA PCR Primer, Index 1 (100% over 32bp)
CGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGAAC484410.42616920503664596RNA PCR Primer, Index 1 (100% over 24bp)
ATGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC446680.3929754970082554RNA PCR Primer, Index 1 (100% over 31bp)
ACGAAGACTGACGCTCAGGTGCGTGGAATTCTCGGGTGCCAAGGAACTCC394480.34705152247653037RNA PCR Primer, Index 1 (100% over 27bp)
TGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGG383810.33766438055596515No Hit
ATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACTCC367410.32323615867243466RNA PCR Primer, Index 1 (100% over 27bp)
TGCGTAGAGATCTGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC366140.3221188512460881RNA PCR Primer, Index 1 (100% over 33bp)
ATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACG365160.32125667701158445No Hit
TCGACACACTATCATTAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG361530.3180631132654128RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGC345050.30356450981171873No Hit
ATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAGGAACTC319770.2813239336400328RNA PCR Primer, Index 1 (100% over 26bp)
ATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGTGGAATTCT276980.24367859129879685No Hit
TGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAAC267790.2355935084262575No Hit
AAACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAA267070.23496007429478546RNA PCR Primer, Index 1 (100% over 23bp)
CGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTC242060.2129570359224015RNA PCR Primer, Index 1 (100% over 26bp)
TAAACGATGTCGACTTGGAGGTTGTGCCCTTGGAATTCTCGGGTGCCAAG229660.2020478925470492No Hit
GATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACTC226080.19889831728222973RNA PCR Primer, Index 1 (100% over 26bp)
AGAACTCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG225440.19833526472092122RNA PCR Primer, Index 2 (100% over 38bp)
ACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTG220340.19384843962299408No Hit
ACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAA175180.15411804326566264No Hit
GCGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC167690.14752856875909903RNA PCR Primer, Index 1 (100% over 24bp)
TCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGGCAAGACG153500.135044637751337No Hit
AACGATGTCGACTTGGAGGTTGTGCCTTGGAATTCTCGGGTGCCAAGGAA151270.13308275148302767RNA PCR Primer, Index 1 (100% over 23bp)
ACCGGAGTGGACGCATCACTGGTGTTCGGGTTGGAATTCTCGGGTGCCAA149680.13168391777602684No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGGAATTCTCGGGTGCCAA147600.1298539969517742No Hit
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCC143210.12599180828904866No Hit
ACGATGTCGACTTGGAGGTGCCCTGGAATTCTCGGGTGCCAAGGAACTCC139680.12288622150558144RNA PCR Primer, Index 1 (100% over 27bp)
TAAGTCGACCGCCTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA134190.11805628625310692RNA PCR Primer, Index 1 (100% over 32bp)
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGTGGAATTCT133610.11754601986942108No Hit
AATGCGTAGAGATCTGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT129860.11424688376800403RNA PCR Primer, Index 1 (100% over 30bp)
ACGATGTCGACTTGGAGGTTGTGCCTTGGAATTCTCGGGTGCCAAGGAAC126930.11166915876076354RNA PCR Primer, Index 1 (100% over 24bp)
TGAGAGAACTCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC121430.10683042581201857RNA PCR Primer, Index 2 (100% over 34bp)
AACGATGTCGACTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC120050.1056163437266971RNA PCR Primer, Index 2 (100% over 34bp)
AGGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC119920.10550197367518131RNA PCR Primer, Index 1 (100% over 24bp)
ATGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAAC118380.10414712844953272RNA PCR Primer, Index 1 (100% over 24bp)
ATGCGTAGAGATCTGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA118130.10392718604277158RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGAGTGGACGCATCACTGGTGTTCGGGTGGAATTCTCGGGTGCCAAG116130.1021676467886825No Hit
AAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGTGGAATTCTCGGGTG115890.10195650207819182No Hit
AGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCATGGAATTCTCGGGT114810.1010063508809837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)